The 69th Seminar and Annual Meeting for Cryobiology and Cryotechnology, 2024：Annual Meetings：ACTIVITIES：JSCC

The 69th Seminar and Annual Meeting for Cryobiology and Cryotechnology, 2024

Date:	June 15(Sat) - 16(Sun), 2024
Venue:	Kochi Kaikan, Hokuho

Seminar Program

Saturday, June 15th, 2024
【Seminar theme: Preservation of mammalian genetic resources by freeze-drying of cells】

10:40-10:45		Opening remarks Keisuke EDASHIGE
（Chairperson: Keisuke EDASHIGE）
10:45-11:25	S1	Preservation of mammalian genetic resources at room temperature by freeze-drying 〇Teruhiko WAKAYAKA （Advanced Biotechnology Center, University of Yamanashi）
11:25-13:00		Lunch break
（Chairperson: Keisuke EDASHIGE）
13:00-13:40	S2	Freeze-drying of mouse and rat sperm, and apply for wild animals 〇Takehito KANEKO （Osaka Metropolitan University）
（Chairperson: Takahiro KIKAWADA）
13:40-14:20	S3	Current status of research aimed at establishing freeze-drying preservation technology for pig sperm 〇Michiko NAKAI, Nguyen Thi MEN, Kazuhiro KIKUCHI （NARO）
14:20-15:00	S4	Utilization and expansion of cell freeze-drying technology in the livestock 〇Kazutsugu MATSUKAWA¹, Takafumi KAMEDA², Toshinori OIKAWA³, Keisuke EDASHIGE¹ （¹Kochi University, ²The United Graduate School of Agricultural Sciences, Ehime University, ³Miyagi Prefectural Livestock Experiment Station）

【Best Presentation nominates (Total 11 titles: 7 in day 1)】

（Chairperson: Kiyoshi KAWAI）
15:10-15:25	B1	Cryopreservation of high concentrated protein in condensed aqueous choline dihydrogen phosphate solutions ☆Masaharu NAKAYAMA, Kenou KAKINUMA, Atsushi YAMADA, Takahiro TAKEKIYO, Yukihiro YOSHIMURA （National Defense Academy）
15:25-15:40	B2	New design of zwitterions as cryoprotectants ☆Takeru ISHIZAKI¹, Kojiro ISHIBASHI², Kenji TAKAHASHI¹, Eishu HIRATA², Kosuke KURODA¹ （¹Faculty of Biological Science and Technology, Institute of Science and Engineering, Kanazawa University, ²Cancer Research Institute, Kanazawa University)
15:40-15:55	B3	Cryoprotective effect of intracellularly introduced glycopolymers ☆Yusuke KATO, Kazuaki MATSUMURA （Japan Advanced Institute of Science and Technology）
（Chairperson: Shinsuke SEKI）
15:55-16:10	B4	Effect of vitrification on global DNA methylation levels and gene expression in bovine embryos ☆Minori SHIDA, Jyun ITO , Shunsuke HARA, Hisataka IWATA （Tokyo University of Agriculture）
16:10-16:25	B5	Investigation of the cryopreservation using AQP4 overexpressing cells: dehydration dependence on viability at various freezing rates ☆Sumire MATSUO¹, Masato YASUI², Youichiro ABE², Tsutomu UCHIDA¹ （¹Hokkaido University, ²Keio University）
16:25-16:40	B6	Raf-like kinase ARK-mediated regulation and the function of ethylene response factors in cold acclimation of the moss Physcomitrium Patens ☆Kanata HIROTA¹, Md. Masudul Karim¹, Yoichi SAKATA², Daisuke TAKEZAWA¹　（¹Saitama University, ²Tokyo University of Agriculture）
16:40-16:55	B7	"Flp/FRT" site-specific recombination system in the anhydrobiotic Pv11 cells ☆Masahiko HOTTA^1,2, Yuki YOSHIDA², Takahiro　KIKAWADA^1,2 (1Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 2Biological Function Research Group, Division of Biomaterial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research　Organization (NARO)
17:20-19:20		Banquet

Sunday, June 16, 2024
【Best Presentation nominates (Total 11 titles: 4 in day 2)】

（Chairperson: Jun KASUGA）
9:30-9:45	B8	*Elucidation of the cell wall-related mechanisms contributing to the desiccation tolerance in Selaginella* species** ☆Momoka OHASHI¹, Takuma KIKUCHI¹, Kimie TSUJI², Yasuko KANEKO³, Toshihisa KOTAKE¹, Daisuke TAKAHASHI¹ （¹Graduate School of Science and Engineering, Saitama University, ²Comprehensive Analysis Center for Science, Saitama University, ³Faculty of Education, Saitama University）
9:45-10:00	B9	Glass transition temperature of calcium lactate ☆Takumi MOCHIZUKI, Kiyoshi KAWAI （Hiroshima University）
10:00-10:15	B10	MRI visualization method for analysis of diversity of freezing　modes in plants ☆Kazuma IWAZAKI¹, Masaya ISHIKAWA², Naoya TAKAGAWA¹, Naoto FUJITA¹, Yasuhiko TERADA¹ （¹University of Tsukuba，²University of Tokyo）
10:15-10:30	B11	A study on the structure and freezing behavior of winter buds of larch ☆Romi TSUTSUI¹, Chika NAKANO², Toshiaki ITO³, Yuzou SANO³, Keita ARAKAWA³ （¹Graduate School of Agriculture, Hokkaido University, ²School of Agriculture, Hokkaido University, ³Research Faculty of Agriculture, Hokkaido University）

【General presentation (Total 9 titles: 3 titles)】

（Chairperson: Satoru KIDOKORO）
10:30-10:45	G1	Interspecific variations in the ice nucleation activity of wild Rosa stems Yoshihiro UEDA¹, Kenichiro ANMA¹, 〇Masaya ISHIKAWA2 （¹Chiba University,²University of Tokyo）
10:45-11:00	G2	Effects of sheet cover on overwintering of grape buds Hayato KANEYA¹, Maki KANAYA², Hakaru AZUMA³, ◯Jun KASUGA¹ （¹Obihiro University of Agriculture and Veterinary Medicine, ²Hokkaido Agriculture Research Center, NARO, ³Tokachi-Ikeda Research Institute for Viticulture and Enology）
11:00-11:15	G3	*Cryopreservation of Ceratopteris* callus and regeneration of plants from cryo-preserved callus** Shun ISHIOKA¹, Ayumi UEHARA², Daisuke TANAKA³, ◯Akira KURIYAMA¹ （¹Tokyo Denki University, ²Tamagawa University, ³NARO）
11:15-12:30		Lunch break
12:30-13:10		Business meeting

【Award ceremony and lecture】


13:10-13:20		JSCC encouragement award ceremony
13:20-13:50		Freezing adaptation in winter buds and cryopreservation of tree genetic resources Keita ENDOH (Forestry and Forest Products Research Institute, Forest Tree Breeding Center)
13:50-14:00		BP award ceremony
14:00-14:10		Coffee break

【General presentation (Total 9 titles: 6 titles) 】

（Chairperson: Kazuaki MATSUMURA）
14:10-14:25	G4	Consideration of ice crystal suppression effect of trehalose in inkjet superflash freezing 〇Yoshitake AKIYAMA, Tomona TAKIGAWA, Hiroki WATANABE （Shinshu University）
14:25-14:40	G5	Cryopreservation experiments of CHO-TRET1 cells using high-pressure vitrification method 〇Tsutomu UCHIDA¹、Yoshiharu SUZUKI²、Kaito SASAKI², Takahiro KIKAWADA^3,4 （¹Hokkaido University, ²Tokai University, ³National Agriculture and Food Research Organization, ⁴The University of Tokyo）
14:40-14:55	G6	Cryopreservation of rat embryos by small-volume vitrification procedure and rapid warming 〇Shinsuke SEKI¹, Toshiaki KAWABE², Kazuaki MATSUMURA³, Misako HIGASHIYA¹, Megumi YANO¹, Takanori OIKAWA¹, Yuriko FUJII¹, Wataru YAMAZAKI¹, Tomoo ETO⁴ (¹Akita University, ²ARK Resource Co., Ltd., ³Japan Advanced Institute of Science and Technology, ⁴Central Institute for Experimental Animals)
14:55-15:10	G7	Organ preservation method using bioactive gases 〇Naoyuki HATAYAMA¹, Shuichi HIRAI², Munekazu NAITO¹ (¹Aichi Medical University Department of Anatomy, ²Nihon University Department of Anatomy)
（Chairperson: Tadanori SEI）
15:10-15:25	G8	Glass to rubber transition of bacteria 〇Kiyoshi KAWAI¹, Tomochika SOGABE1, Hiroshi NAKAGAWA², Takeshi YAMADA³, Shigenobu KOSEKI⁴ （¹Graduate School of Integrated Sciences for Life, Hiroshima University, ²Materials Sciences Research Center, Japan Atomic Energy Agency, ³Neutron Science and Technology Center, Comprehensive Research Organization for Science and Society, ⁴Research Faculty of Agriculture, Hokkaido University）
15:25-15:40	G9	Evaluation of endogenous trehalose content and equilibrium moisture content on the glassy state of Artemia dormant eggs Tsukasa SAKO^1,2, Miki MOROI¹, ○Toru Yoshida1 (¹Mukogawa Women's University, ²Current Affiliation: Japan Food Research Laboratories)
15:40-15:45		Closing remarks Keisuke EDASHIGE

ACTIVITIES ACTIVITIES

The 69th Seminar and Annual Meeting for Cryobiology and Cryotechnology, 2024

Seminar Program

ACTIVITIES
ACTIVITIES