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The 58th Annual Meeting of Japanese Society for Cryobiology and Cryotechnology, 2013

The 58th Annual Meeting of Japanese Society for Cryobiology and Cryotechnology, 2013

When:	June 22 and 23, 2013
Where：	Kansai University

June 22 Seminar

9:50～10:40	What do the bacteria do in the environment, plus or minus ○Yoshimi Suzuki, Casareto E. Beatriz (Graduate School of Science and Technology, Shizuoka University)
10:40～11:30	Even if ice nucleation bacteria freeze, why are they valid? Hidehisa Kawahara（Kansai University）
11:30～12:20	Atmospheric Icing-Present Problems and Measures for Prevention - ○Shigeo KIMURA1, Hirotaka SAKAUE2, Katsuaki MORITA2, Taro TANAKA3, Yoichi YAMAGISHI1 (1Kanagawa Institute of Technology, 2Japan Aerospace Exploration Agency, 3Fuji Heavy Industries Ltd.)
13:30～14:20	Control of nucleation and growth of ice inspired by antifreeze protein Takaaki Inada（National Institute of Advanced Industrial Science and Technology (AIST)）

Presentations of Annual Meeting

14:45～15:00	Functional Analysis of Antifreeze Protein from Ascomycete ○Daichi Fukami1, Yuichi Hanada1, Jing Cheng1, Sakae Tsuda1,2, Hidemasa Kondo1,2(1Transdiscriplinary Life Science Course, Graduate School of Life Science, Hokkaido University (HU). ,2 Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).)
15:00～15:15	Estimation of Appropriate Cryopreservation Agents in Cryopreservation at Cell Adhesion State ○H. Sekine，N. Murase，Y. Nagahara(Graduate School of Tokyo Denki University Department of Science and Engineering)
15:15～15:30	Elucidation of structural base of fungal antifreeze protein ○Jing Cheng1,2, Yuichi Hanada1,2, Hidemasa Kondo1,2, and Sakae Tsuda1,2 (1 Graduate School of Life Science, Hokkaido University, 2 Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)1)
15:30～15:45	Interaction of light and temperature in cold acclimation process in plants ○Hiroyuki Imai1, Juri Yakuwa1, Yukio Kawamura1, Akira Nagatani2, and Matsuo Uemura1 (1Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, 2Graduate School of Science, Kyoto University)
15:45～16:00	*Temporal Analysis of Plasma Membrane Proteome in Arabidopsis* during Cold Acclimation and Deacclimation** ○Yushi MIKI1, Daisuke TAKAHASHI2, Yukio KAWAMURA1, 2, Matsuo UEMURA1, 2（1Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University and 2United Graduate School of Agricultural Science, Iwate University）
16:00～16:15	Structure and Activity of Lysozyme in the Ionic Liquid-Water Mixed Solutions: Protein Preserving Solvent at Low-Temperature ○Machiko Shigemi, Takahiro Mori, Kumiko Yamazaki, Takahiro Takekiyo, Hiroshi Abe, Yukihiro Yoshimura(1 Department of Applied Chemistry, National Defense Academy,2Department of Materials Science and Engineering, National Defense Academy)
16:15～16:30	Relationship between water status, related gene expression and its quality in rice seedｓ during ripening stage under the different temperature treatments before/after heading stages. ○Masayuki Miyazaki1,2, Yumena Ito1, Nong Thi Hue 1, Yushi Ishibashi1, Takashi Yuasa13and Mari Iwaya-Inoue (1Fac. Agr., Kyushu Univ., 2 Fukuoka Agr. Res. Cent. 3Fac. Agr., Miyazaki Univ )
16:45～17:00	Cryoprotective polyampholytes hydrogel Kazuaki Matsumura(School of Materials Science, Japan Advanced Institute of Science and Technology)
17:00～17:15	A novel method to extend preserve bone marrow cells and their progenitors using repetitive freeze-thaw-culture in vivo and in vitro. ○S. Sumida1, T. Kitamura1, N. Motomura2, A. Saito2, Evan Hagen3, Erik Woods3 and Arthur W. Rowe4 (1Sajio Sumida Clinic, and Lab Cryomedicine and Blood Transfusion, Takeda Bldg, 2Tissue Bank, Dept. Cardiovas. Surg, Tokyo Univ. Sch. Med., 3Cook General BioTechnology, 4New York University School of Medicine)
17:15～17:30	Temperature dependence on synchronized beating of neonatal rat ventricular myocytes measured on multi-electrode array Ryuichi Kitora, Shin’ya Taniwaki, Masafuni Nagayama, Kazutoshi Gohara, ○Tsutomu Uchida (Biophysics group, Division of Applied Physics, Faculty of Engineering, Hokkaido University)
17:30～17:45	Effect of annealing and controlled nucleation on physical property of freeze-dried pharmaceutical formulations ○Ken-ichi Izutsu, Chikako Yomota, Yukihiro Goda, Haruhiro Okuda(National Institute of Health Sciences)

June 23

9:00～9:15	Effects of supercooling-facilitating (anti-ice nucleation) substances in various solutions ○Chikako KUWABARA, Keita Endoh, Keita ARAKAWA and Seizo FUJIKAWA (Research Faculty and Graduate School of Agriculture, Hokkaido University)
9:15～9:30	Exploration of ice nucleation activity in plant tissues. ○Masaya Ishikawa　(National Institute of Agrobiological Sciences.)
9:30～9:45	Functional roles of ice nucleation activity in blueberry stems in the freeze initiation, extracellular freezing and survival. H. Yamazaki, ○M. Ishikawa, Y. Nakatani (National Institute of Agrobiological Sciences)
9:45～10:00	*Ice segregation phenomenon by Keisukea japonica* Miq. Lamiacea** ○Kazuo Takeda1, Uzuki Matsushima2, Karen Tanino3 (1Obihiro University of Agriculture and Veterinary Medicine, 2Iwate University, 3University of Saskatchewan)
10:00～10:15	*Water pathway in xylem during ice segregation by Keisukea japonica* Miq.** ○1Uzuki Matsushima, 2Kazuo Takeda , 3Karen K. Tanino, Karen Tanino3　(1Iwate University, 2Obihiro University of Agriculture and Veterinary Medicine, 3University of Saskatchewan)
10:15～10:30	Protective effects of flavonoid glycosides against low-temperature induced cell injury ○Fuminori Kato1, Tomoko Kawagoe1, Norifusa Matsuo1, Seizo Fujikawa2　(1Central Research Institute of Ishihara Sangyo Kaisha, Ltd., 2Professor emeritus in Hokkaido University)
10:45～11:00	Rice plants sense today’s weather condition and regulate aquaporin gene expression in the roots. ○Mari Murai-Hatano1, Tsuneo Kuwagata2, Junko Ishikawa-Sakurai1, Hidehiro Hayashi1(1NARO Agricultural Research Center for Tohoku Region, 2 National Institute for Agro-Environmental Sciences (NIAES))
11:00～11:15	Development of in vivo investigation system for the regulation of cold-responsive genes in Arabidopsis Yoko Tominaga, ○Yukio Kawamura, Matsuo Uemura (Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University)
11:15～11:30	Role of endogenous ABA in cold and hyperosmotic stress response of Physcomitrella patens ○Masashi Saruhashi1, Totan Ghosh 1, Atsushi Suzuki1, Naoki Watanabe1, Shinnosuke Somemiya1, Kanako Ishiyama3, Masatomo Kobayashi3, Yoichi Sakata2, Daisuke Takezawa1(1Graduate School of Science and Engineering, Saitama University,2Graduate School of Agriculture, Tokyo University of Agriculture,3RIKEN BioResource Center 1)
11:30～11:45	Compositional changes in soluble proteins of tree buds in endodormancy release ○Shingo SUZUKI1, Daisuke TAKAHASHI2, Chikako KUWABARA3, Matsuo UEMURA2, Keita ARAKAWA3 (1Graduate School of Agriculture, Hokkaido University, 2Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, Research Faculty of Agriculture, Hokkaido University )
11:45～12:00	Culture of Protoplasts Isolated from Desiccation Tolerance Acquired Carrot Suspension Cells ○M. Mulati 1 , H. Matsuo 1 , ?M. Yamaguchi2, A. Kuriyama 1 , N. Murase 1 (1Graduate School of Science and Engineering, Tokyo Denki University, 2Graduate School of Advanced Science and Engineering, Tokyo Denki University )
12:00～12:15	Desiccation tolerance and long-term storage after desiccation of cultured cells of Citrus ○Yasutake Sugawara1),Kyosuke Mukae2),Rie Hatanaka3),Hisato Kunitake4) (1)Saitama University, 2)Graduate School of Science and Technology, Saitama University,3)National Institute of Agrobiological Science, 4)Faculty of Agriculture, University of Miyazaki)
12:15～12:30	Preservation of cucumber using an osmotic dehydrofreezing technique Naoto Mamada1, Takuya Fukatsu1, Ryuichi Hata2, ○Kazuhito Kajiwara1,2(1School of Bioscience and Biotechnology, 2Tokyo University of Technology )
12:35～13:35	Interuniversity Bio-Backup Project for Life Science ○Daisuke Tanaka（IBBP Center, National Institute for Basic Biology）
14:10 ～14:15	Award Ceremony
14:15～14:45	Awardee’s Special Presentation Cold acclimation and freezing injury in plants Matsuo Uemura (Cryobiofronteir Research Center, Faculty of Agriculture, Iwate University)
14:45～15:05	Awardee’s Special Presentation Study on glass transition properties of biomaterials and its application Kiyoshi Kawai (Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University)

Annual Meeting

15:15～15:30	Anti-aggregation Effects on Liposomes during Desiccation by Model Peptides of Group3 LEA Proteins ○Takao Furuki1, Minoru Sakurai1 (1 BioCenter of Tokyo Tech.)
15:30～15:45	Assessment for anti-aggregation activity of a LEA protein by dynamic light scattering ○Takahiro Kikawada, Rie Hatanaka (Anhydrobiosis research Group, National Institute of Agrobiological Sciences)
15:45～16:00	Experimental study of the mechanism by which desiccation-induced protein aggregation is suppressed by LEA peptide K.Yamakawa1, T.Furuki1, T.Furut1, R.Hatanaka2, T.Kikawada2, T.Niwa2, H.Taguchi2, H.Furusawa2, Y.Okahata2, ○M.Sakurai1 (1BioCenter of Tokyo Tech., 2National Institute of Agrobiological Science, 3 Dept.Biomolecular Engineering of Tokyo Tech.)
16:00～16:15	Computational study of the mechanism by which desiccation-induced protein aggregation is suppressed by LEA peptide K. Yamakawa1, T. Furuki1, T. Furuta1, ○M. Sakurai1(1 BioCenter of Tokyo Tech.)
16:30～16:45	*Evaluation of freezing damages after cryopreservation using decapsulated A. franciscana* cysts.** ○Toru Yoshida1, Midori Tanaka1, Mari Sakaue2, Keisuke Fukuo1, and Mitsuru Fukuda1(1 Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University, 2 Science & Medical Systems Business Group, Hitachi High-Technologies Corporation)
16:45～17:00	Cryopreservation technique using a micro droplet ○Norihito Kimizuka, Kinzi Watanabe (School of Food, Agricultural and Environmental Sciences Miyagi University )
17:00～17:15	High quality concentration of liquid food by progressive freeze-concentration ○Osato Miyawaki, Mihiri Gunathilake, and Kiyomi Shimmura (Department of Food Science, Ishikawa Prefectural University)
17:15～17:30	Proposal of Mathematical Modeling of Cell Death due to Slow-freezing Injury　(Development of Reaction Kinetic Model for Cell Survival Curve as a Function of End Temperature) ○1Hiroshi ISHIGURO, 2Masakazu NOZAWA(1Department of Biological Functions and Engineering,Graduate School of Life Science and Systems Engineering,Kyushu Institute of Technology,2 Department of Mechanical Engineering, Akita National College of Technology)
17:30～17:45	Elastic and Thermal Properties of Liquid-glass Transitions in Ternary Mixtures of Water, Sugar and an Ionic Liquid ○Haruki Takayama, Tomohiko Shibata, Yuto Kuroda, Shota Koda and Seiji Kojima(Graduate School of Pure and Applied Sciences, University of Tsukuba)