１．原著論文/総説（責任著者*）

[2024年]
Kubohara Y*, Fukunaga Y, Shigenaga A, & Kikuchi H.
Dictyostelium differentiation-inducing factor 1 promotes glucose uptake via direct inhibition of mitochondrial malate dehydrogenase in mouse 3T3-L1 cells.
Int. J. Mol. Sci.25(3), 1889. (2024).

Yoshida N, Kikuchi H, Hirai M, Balikagala B, Anywar DA, Taka H, Kaga N, Miura Y, Fukuda N, Odongo-Aginya EI, Kubohara Y*, & Mita T*.
A longer-chain acylated derivative of Dictyostelium differentiation-inducing factor-1 enhances the antimalarial activity against Plasmodium parasites.
Biochem. Pharmacol. 225, 116243. (2024).
国内&海外プレスリリース;
https://en.juntendo.ac.jp/highlights/news/nid403
https://www.eurekalert.org/news-releases/1051917

Nishimura T*, Murotani T, Sasaki H, Uekusa Y, Eguchi H, Ishigaki H, Takahashi K, Kubohara Y, & Kikuchi H*.
Isolation and structure determination of new pyrones from Dictyostelium spp. cellar slime molds coincubated with Pseudomonas spp.
Molecules, 29(9), 2143. (2024).

久保原禅*（和文総説）
誰も知らないマイナー生物の研究も世の役に立つ！〜粘菌は未開拓創薬資源!?〜
J. Health & Sports Sci. Juntendo（順天堂スポーツ健康科学研究）14(1), 46-50. (2024).


[2023年]
Hirayama A, Ishigaki H, Takahashi K, Miura Y, Kikuchi H, & Kubohara Y*.
Dictyostelium differentiation-inducing factor derivatives reduce the glycosylation of PD-L1 in MDA-MB-231 human breast cancer cells.
Juntendo Medical J. 69(2), 105-115. (2023).

Kuwayama H, Kikuchi H, & Kubohara Y*.
Derivatives of differentiation-inducing factor 1 differentially control chemotaxis and stalk cell differentiation in Dictyostelium discoideum.
Biology, 12(6), 873. (2023).

Kubohara Y*, Fukunaga Y, Kikuchi H, & Kuwayama H.
Pharmacological evidence that Dictyostelium differentiation-inducing factor 1 promotes glucose uptake partly via an increase in intracellular cAMP content in mouse 3T3-L1 cells.
Molecules, 28(23), 7926. (2023).

[2021年]
Mita T*, Hirai M, Maki Y, Nahar S, Ikeda M, Yoshida N, Oshima Y, Kikuchi H, & Kubohara Y*.
Derivatives of Dictyostelium differentiation-inducing factors suppress the growth of Plasmodium parasites in vitro and in vivo.
Biochem. Pharmacol.(2021). In press.

Kubohara Y*, Homma Y, Shibata H, Oshima Y, Kikuchi H.
Dictyostelium differentiation-inducing factor-1 promotes glucose uptake, at least in part, via an AMPK-dependent pathway in mouse 3T3-L1 cells.
Int. J. Mol. Sci. 22(5), 2293. (2021).

飯泉恭一*、重永綾子、都築孝允、川崎広明、山倉文幸、久保原禅
炎症性腸疾患の制御に関わる分子の探索－動物モデルを用いた運動の効果－
東北女子大学紀要 59, 15-19. (2021).

[2020年]
Sasaki H, Kubohara Y, Ishigaki H, Takahashi K, Eguchi H, Sugawara A, Oshima Y, & Kikuchi H*.
Novel two terpenes isolated from Dictyostelium cellular slime molds.
Molecules, 25(12), 2895. (2020).

[2019年]
Fujita K, Tanaka S, Iizumi K, Akiyama S, Uchida K, Ogata M, Aoki D, Hosomi O*, & Kubohara Y*.
Melbiosamine, a novel oligosaccharide, suppresses mitogen-induced IL-2 production via inactivation of NFAT and NFκB in Jurkat cells.
Biochem. Biophys. Rep. 19, 100658. (2019).

Totsuka K, Makioka Y, Iizumi K, Takahashi K, Oshima Y, Kikuchi H*, & Kubohara Y*.
Halogen-substituted derivatives of Dictyostelium differentiation-inducing factor-1 suppress serum-induced cell migration of human breast cancer MDA-MB-231 cells in vitro.
Biomolecules9(7), 256. (2019).

Kubohara Y*, Shiratsuchi Y, Ishigaki H, Takahashi K, Oshima Y, & Kikuchi H.
Antimicrobial activities of Dictyostelium differentiation-inducing factors and their derivatives.
Biomolecules 9(5), 163. (2019).

Kubohara Y*, Kikuchi H, & Oshima Y.
Derivatives of Dictyostelium differentiation-inducing factors inhibit serum-dependent cell migration of murine osteosarcoma LM8 cells.
Juntendo Med. J.65(1), 71-76 (2019).

Kubohara Y*, & Kikuchi H. (Invited Review)
Dictyostelium: an important source of structural and functional diversity in drug discovery.
Cells 8(1), 6. (2019).

[2018年]
Iizumi K, Kawasaki H, Shignaga A, Tominaga M, Otsu A, Kamo A, Kamata Y, Takamori K, & Yamakura F*.
Tryptophan nitration of immunoglobulin light chain as a new possible biomarker for atopic dermatitis.
J. Clin. Biochem. Nutr. 63(3), 197-204. (2018).

中里隆之佑、飯泉恭一、佐々木啓、重永綾子、尾形慎、青木大地、 三澤義知、細見修、久保原禅*（和文）
Effects of a novel oligosaccharide, MelNH2, on human breast cancer cells
（ヒト乳がん細胞の増殖と遺伝子発現に対する新規オリゴ糖MelNH2の効果）
J. Health & Sports Sci. Juntendo（順天堂スポーツ健康科学研究）9(1), 1-10. (2018).

[2017年]
Takahashi K, Kikuchi H*, Nguyen VH, Oshima Y, Ishigaki H, Nakajima-Shimada J, & Kubohara Y*.
Biological activities of novel derivatives of differentiation-inducing factor-3 from Dictyostelium discoideum.
Biol. Pharm. Bull. 40(11), 1941-1947. (2017).

Kikuchi H*, Ito I, Takahashi K, Ishigaki H, Iizumi K, Kubohara Y, & Oshima Y.
Isolation, synthesis and biological activity of chlorinated alkylresorcinols from Dictyostelium cellular slime molds.
J. Nat. Prod. 80(10), 2716-2722. (2017).

Kubohara Y*, Kikuchi H, Nguyen VH, Kuwayama H, & Oshima Y.
Evidence that differentiation-inducing factor-1 controls chemotaxis and cell differentiation, at least in part, via mitochondria in Dictyostelium discoideum.
Biology Open 6, 741-751. (2017).

Nguyen VH, Kikuchi H*, Sasaki H, Iizumi K, Kubohara Y, & Oshima Y.
Production of novel bispyrone metabolites in the cellular slime mold Dictyostelium giganteum induced by zinc(II) ion.
Tetrahedron 73(5), 583-588. (2017).

[2016年]
Kuwayama H, Kikuchi H, Oshima Y, & Kubohara Y*.
Glutathione S-transferase 4 is a putative DIF-binding protein that regulates the size of fruiting bodies in Dictyostelium discoideum.
Biochem. Biophys. Rep. 8, 219-226. (2016).

Zhang J*, Yamada O*, Kida S, Matsushita Y, Murase S, Hattori T, Kubohara Y, Kikuchi H, & Oshima Y.
Identification of brefelamide as a novel inhibitor of osteopontin that suppresses invasion of A549 lung cancer cells.
Oncol. Rep.36(4), 2357-2364. (2016).

Kawaharada R, Nakamura A, Takahashi K, Kikuchi H, Oshima Y, & Kubohara Y*.
Oral administration of Dictyostelium differentiation-inducing factor 1 lowers blood glucose levels in streptozotocin-induced diabetic rats.
Life Sci. 155, 56-62. (2016).

Kuwayama H, & Kubohara Y*.
Differentiation-inducing factor 2 modulates chemotaxis via the histidine kinase DhkC-dependent pathway in Dictyostelium discoideum.
FEBS Lett. 590(6), 760-768. (2016).

[2015年]
Oladimeji P, Kubohara Y, Kikuchi H, Oshima Y, Rusch C, Skerl R, & Diakonova M*.
A Derivative of Differentiation-Inducing Factor-3 Inhibits PAK1 Activity and Breast Cancer Cell Proliferation.
Int. J. Cancer Clinic. Res. 2(3), 1-6. (2015).

Nguyen VH, Kikuchi H*, Kubohara Y, Takahashi K, Katou Y, & Oshima Y*.
Development of novel DIF-1 derivatives that selectively suppress innate immune responses.
Bioorg. Med. Chem. 23(15), 4311-4315. (2015).

Kubohara Y*, Komachi M, Homma Y, Kikuchi H, & Oshima Y.
Derivatives of Dictyostelium differentiation-inducing factors inhibit lysophosphatidic acid–stimulated migration of murine osteosarcoma LM8 cells.
Biochem. Biophys. Res. Commun.463(4), 800-805. (2015).

Uda M, Kawasaki H, Iizumi K, Shigenaga A, Baba T, Naito H, Yoshioka T, Yamakura F*.
Sumoylated α-skeletal muscle actin in the skeletal muscle of adult rats.
Mol Cell Biochem. 409(1-2):59-66. (2015).

[2014年]
Kubohara Y*, Kikuchi H, Matsuo Y, Oshima Y, & Homma Y.
Properties of a non-bioactive fluorescent derivative of differentiation-inducing factor-3, an anti-tumor agent found in Dictyostelium discoideum.
Biology Open. 3(4), 289-296. (2014).

Kawasaki H, Tominaga M, Shigenaga A, Kamo A, Kamata Y, Iizumi K, Kimura U, Ogawa H, Takamori K, Yamakura F*.
Importance of tryptophan nitration of carbonic anhydrase III for the morbidity of atopic dermatitis.
Free Radic Biol Med. 73:75-83.(2014)

[2013年]
Nakajima-Shimada J*, Hatabu T, Hosoi Y, Onizuka Y, Kikuchi H, Oshima Y, & Kubohara Y*.
Derivatives of Dictyostelium discoideum differentiation-inducing factor-3 suppress the activities of Trypanosoma cruziin vitro and in vivo.
Biochem. Pharmacol. 85(11), 1603-1610. (2013).
プレスリリース：群馬テレビニュースで放送、朝日新聞・読売新聞・毎日新聞・産経新聞・上毛新聞に記事掲載

Kikuchi H*, Kubohara Y, Nguyen VH, Katou Y, & Oshima Y.
Novel chlorinated dibenzofurans Isolated from the cellular slime mold, Polysphondylium filamentosum, and their biological activities.
Bioorg. Med. Chem. 21(15), 4628-4633. (2013).

Yamada Y, Kubohara Y, Kikuchi H, Oshima Y, Wang HY, Ross S, & Williams JG*.
The Dictyostelium prestalk inducer DIF-1 directs phosphorylation of a bZIP transcription factor.
Int. J. Dev. Biol. 57(5), 375-381. (2013).

Kubohara Y*, Kikuchi H, Matsuo Y, Oshima Y, & Homma Y.
Mitochondria are the target organelle of differentiation-inducing factor-3, an anti-tumor agent isolated from Dictyostelium discoideum.
PLoS ONE. 8(8), e72118. (2013).
プレスリリース：読売新聞・産経新聞・日経新聞・上毛新聞などに記事掲載

[2012年]
Seya K*, Kanemaru K, Matsuki M, Hongo K, Kitahara H, Kikuchi H, Oshima Y, Kubohara Y, Okumura K, Motomura S, & Furukawa K.
Br-DIF-1 accelerates dimethyl sulphoxide-induced differentiation of P19CL6 embryonic carcinoma cells into cardiomyocytes.
Brit. J. Pharmacol. 165(4), 870-879. (2012).

Tanaka S, Masuda Y, Honma C, Hosaka K, Takahashi K, & Kubohara Y*.
Manganese promotes phorbol ester–induced interleukin-2 production via AP-1 activation in Jurkat T-cells.
Toxicol. Lett. 211(3), 312-318. (2012).

Kikuchi H*, Matsuo Y, Katou Y, Kubohara Y, & Oshima Y.
Isolation, synthesis and biological activity of biphenyl and m-terphenyl-type compounds from Dictyostelium cellular slime molds.
Tetrahedron 68(43), 8884-8889. (2012).

久保原禅*（和文総説）
細胞性粘菌由来の生物活性物質DIF-1, DIF-2, DIF-3
和光純薬時報80(2), 2-6. (2012).

[2011年]
Takahashi K, Murakami M*, Kikuchi H, Oshima Y, & Kubohara Y*.
Derivatives of Dictyostelium differentiation-inducing factors promote mitogen-activated IL-2 production via AP-1 in Jurkat cells.
Life Sci. 88(11-12), 480-485. (2011).

Kuwayama H, Kikuchi H, Oshima Y, & Kubohara Y*.
Artificial compounds differentially control Dictyostelium chemotaxis and cell differentiation.
Cell Structure and Function.36(1), 21-26. (2011).

Luciani M-F, Giusti C, Harms B, Oshima Y, Kikuchi H, Kubohara Y, & Golstein P*.
Atg1 allows second-signalled autophagic cell death in Dictyostelium.
Autophagy 7(6), 501-508. (2011).

[2010年]
Matsuda T, Takahashi-Yanaga F*, Yoshihara T, Maenaka K, Watanabe Y, Miwa Y, Morimoto S, Kubohara Y, Hirata M, & Sasaguri T.
Dictyostelium differentiation-inducing factor-1 binds to mitochondrial malate dehydrogenase and inhibits its activity.
J. Pharmacol. Sci. 112(3), 320-326. (2010).

Kubohara Y*, Kikuchi H, Nakamura K, Matsuo Y, & Oshima Y.
Preparation of an antibody that recognizes and neutralizes Dictyostelium differentiation-inducing factor-1.
Biochem. Biophys. Res. Commun. 396(2), 364-369. (2010).

Kikuchi H*, Ishiko S, Nakamura K, Kubohara Y, & Oshima Y*.
Novel prenylated and geranylated aromatic compounds isolated from Polysphondylium cellular slime molds.
Tetrahedron 66(32), 6000-6007. (2010).

Calvo-Garrido J, Carilla-Latorre S, Kubohara Y, Santos N, Mesquita A, Soldati T, Golstein P, & Escalante R*. (Review)
Autophagy in Dictyostelium : genes and pathways, cell death and infection.
Autophagy 6(6), 686-701. (2010).

久保原禅*、桑山秀一（和文総説）
細胞性粘菌の細胞分化と走化性運動を制御する低分子化合物
生化学 82(12), 1132-1137. (2010).

[2009年]
Hosomi O*, Misawa Y, Takeya A, Matahira Y, Sugahara K, Kubohara Y, Yamaura F, & Kudo S.
Novel oligosaccharide has suppressive activity against human leukemia cell proliferation.
Glycoconjugate J. 26(2), 189-198. (2009).

Kikuchi H*, Hoshi T, Kitayama M, Sekiya M, Katou Y, Ueda K, Kubohara Y, Sato H, Shimazu M, Kurata S, & Oshima Y*.
New diterpene pyrone-type compounds, Metarhizin A and B, isolated from entomopathogenic fungus, Metarhizium flavoviride and their structural requirements for inhibiting cellular proliferation.
Tetrahedron. 65(2), 469-477. (2009).

Luciani M-F, Kubohara Y, Kikuchi H, Oshima Y, & Golstein P*.
Autophagic or necrotic cell death triggered by distinct motifs of the differentiation factor DIF-1.
Cell Death & Differ. 16(4), 564-570. (2009).

Takahashi K, Murakami M*, Hosaka K, Kikuchi H, Oshima Y, & Kubohara Y*.
Regulation of IL-2 production in Jurkat cells by Dictyostelium-derived factors.
Life Sci. 85(11-12), 438-443. (2009).

Kuwayama H, & Kubohara Y*.
Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxis.
PLoS ONE. 4(8), e6658. (2009).
Selected for Faculty of 1000 Biology (2009).
プレスリリース：NHKニュース・群馬テレビニュースで放送、朝日新聞・上毛新聞・信濃毎日新聞他数紙に記事掲載

[2008年]
Kubohara Y*, Kikuchi H, & Oshima Y.
Exploitation of the derivatives of Dictyostelium differentiation-inducing factor-1, which promote glucose consumption in mammalian cells.
Life Sci. 83(17-18), 608-612. (2008).

Kikuchi H, Ishiko S, Oshima Y, Gokan N, Hosaka K, & Kubohara Y*.
Biological activities of novel derivatives of DIF-1 isolated from Dictyostelium.
Biochem. Biophys. Res. Commun.377(3), 1012-1017. (2008).

保坂公平、高橋克典、久保原禅*（和文）
Calcineurin inhibitors present in plastic and rubber products.（プラスチック・ゴム製品中に含まれるカルシニューリン阻害物質）
Jpn. J. Environ. Toxicol.（日本環境毒性学会誌）11(2), 143-149. (2008).\

Yamano Y*, Miyakawa S, Iizumi K, Itoh H, Iwasaki M, Tsugane S, Kagawa J, Nakadate T.
Long-term study of urinary bisphenol A in elementary school children.
Environ Health Prev Med. 13(6):332-7. (2008)

[2007年]
Kubohara Y*, Arai A, Gokan N, & Hosaka K.
Pharmacological evidence that stalk cell differentiation involves increases in the intracellular Ca2+and H+ concentrations in Dictyostelium discoideum.
Develop. Growth & Differ.49(3), 253-264. (2007).

Omata W, Shibata H, Nagasawa M, Kojima I, Kikuchi H, Oshima Y, Hosaka K, & Kubohara Y*.
Dictyostelium differentiation-inducing factor-1 induces glucose transporter 1 translocation and promotes glucose uptake in mammalian cells.
FEBS Journal. 274(13), 3392-3404. (2007).
日経産業新聞に成果を紹介する記事掲載

Kikuchi H*, Nakamura K, Kubohara Y, Gokan N, Hosaka K, Maeda Y, & Oshima Y*.
Dihydrodictyopyrone A and C: New members of dictyopyrone family isolated from Dictyostelium cellular slime molds.
Tetrahedron Lett. 48(33), 5905-5909. (2007).

Nara T*,Iizumi K, Ohmae H, Sy OS, Tsubota S, Inaba Y, Tsubouchi A, Tanabe M, Kojima S, Aoki T.
Antibody isotype responses to paramyosin, a vaccine candidate for schistosomiasis, and their correlations with resistance and fibrosis in patients infected with Schistosoma japonicum in Leyte, The Philippines.
Am J Trop Med Hyg. 76(2):384-91. (2007)

久保原禅*（和文総説）
細胞性粘菌の分化誘導因子DIF-1が示す薬理作用：下等生物（粘菌）は面白い
生化学 79(2), 148-151. (2007).

[2006年]
Kikuchi H, Oshima Y, Ichimura A, Gokan N, Hasegawa A, Hosaka K, & Kubohara Y*.
Anti-leukemic activities of Dictyostelium secondary metabolites: A novel aromatic metabolite, 4-methyl-5-n-pentylbenzene-1,3-diol, isolated from Dictyostelium mucoroides suppresses cell growth in human leukemia K562 and HL-60 cells.
Life Sci. 80(2), 160-165. (2006).

Iizumi K, Mikami Y, Hashimoto M, Nara T, Hara Y, Aoki T*.
Molecular cloning and characterization of ouabain-insensitive Na(+)-ATPase in the parasitic protist, Trypanosoma cruzi.
Biochim Biophys Acta. 1758(6):738-46. (2006)

[2005年]
Tanaka S, Akaishi E, Hosaka K, Okamura S, & Kubohara Y*.
Zinc ions suppress mitogen-activated interleukin-2 production in Jurkat cells.
Biochem. Biophys. Res. Commun. 335(1), 162-167. (2005).

Gokan N, Kikuchi H, Nakamura K, Oshima Y, Hosaka K, & Kubohara Y*.
Structural requirements of Dictyostelium differentiation-inducing factors for their stalk-cell-inducing activity in Dictyostelium cells and anti-proliferative activity in K562 human leukemic cells.
Biochem. Pharmacol. 70(5), 676-685. (2005).

Arai A, Goto Y, Hasegawa A, Hosaka K, Kikuchi H, Oshima Y, Tanaka S, & Kubohara Y*.
Dictyopyrones, novel a-pyronoids isolated from Dictyostelium spp., promote stalk cell differentiation in Dictyostelium discoideum.
Differentiation. 73(7), 377-384. (2005).

Kikuchi H, Saito Y, Sekiya J, Okano Y, Saito M, Nakahata H, Kubohara Y, & Oshima Y*.
Isolation and synthesis of a new aromatic compound, brefelamide, from Dictyostelium cellular slime molds and its inhibitory effect on proliferation of astrocytoma cells.
J. Org. Chem. 70(22), 8854-8858. (2005).

[2004年]
Akaishi E, Narita T, Kawai S, Miwa Y, Sasaguri T, Hosaka K, & Kubohara Y*.
Differentiation-inducing factor-1-induced growth arrest of K562 leukemia cells involves the reduction of ERK1/2 activity.
Eur. J. Pharmacol. 485(1-3), 21-29. (2004).

Shimizu K, Murata T, Tagawa T, Takahashi K, Ishikawa R, Abe Y, Hosaka K, & Kubohara Y*.
Calmodulin-dependent cyclic nucleotide phosphodiesterase (PDE1) is a pharmacological target of differentiation-inducing factor-1, an anti-tumor agent isolated from Dictyostelium.
Cancer Research. 64(7), 2568-2571. (2004).

Kikuchi H*, Sasaki K, Sekiya J, Maeda Y, Amagai A, Kubohara Y, & Oshima Y.
Structural requirements of dictyopyrones isolated from Dictyostelium spp. in the regulation of Dictyostelium development and in anti-leukemic activity.
Bioorg. Med. Chem. 12(12), 3203-3214. (2004).

Kubohara Y*, Arai A, Takahashi K, Hosaka K, & Okamoto K.
Prespore-to-stalk conversion involves the production of a pathway-specific glycoprotein, wst25, in the cellular slime mould Dictyostelium discoideum.
Biochem. Biophys. Res. Commun. 320(2), 468-473. (2004).

[2003年]
Kanai M, Konda Y*, Nakajima T, Izumi Y, Nanakin A, Kanda N, Kubohara Y, & Chiba T.
Differentiation-inducing factor-1 (DIF-1) inhibits STAT3 activity involved in gastric cancer cell proliferation via MEK-ERK dependent pathway.
Oncogene. 22(4), 548-554. (2003).

Kubohara Y*, Hanaoka Y, Akaishi E, Kobayashi H, Maeda M, & Hosaka K.
DIF-1, an anti-tumor agent found in Dictyostelium discoideum, inhibits progesterone-induced oocyte maturation in Xenopus laevis.
Eur. J. Pharmacol. 460(2-3), 93-98. (2003).

Takahashi-Yanaga F*, Taba Y, Miwa Y, Kubohara Y, Watanabe Y, Hirata M, Morimoto S, & Sasaguri T.
Dictyostelium differentiation-inducing factor-3 activates glycogen synthase kinase-3b and degrades cyclin D1 in mammalian cells.
J. Biol. Chem. 278(11), 9663-9670. (2003).

Takahashi K, Akaishi E, Abe Y, Ishikawa R, Tanaka S, Hosaka K, & Kubohara Y*.
Zinc inhibits calcineurin activity in vitro by competing with nickel.
Biochem. Biophys. Res. Commun. 307(1), 64-68. (2003).

[2002年]
Kuno M, Shimakura M, Furuta Y, Terashima N, Hosaka K, & Kubohara Y*.
Effects of differentiation-inducing factor-1 (DIF-1) of Dictyostelium discoideum on bacteria, fungi, and an influenza virus.
Kitakanto Med. J.52(4), 253-256. (2002).

[2001年]
Fujimaki S, Kubohara Y, Kobayashi I, & Kojima I*.
Caspase-independent apoptosis induced by differentiation-inducing factor of Dictyostelium discoideum in INS-1 cells.
Eur. J. Pharmacol. 421(2), 93-100. (2001).

Kanno T, Kubohara Y, Goto F, Takata K, & Hayashi K*.
NMDA-receptor-dependent and -independent cytotoxic effects of D. discoideum differentiation-inducing factor-1 on rat cortical neurons.
Develop. Growth & Differ. 43(6), 709-716. (2001).

[2000年]
Miwa Y, Sasaguri T*, Kosaka C, Taba Y, Ishida A, Abumiya T, & Kubohara Y.
DIF-1, a morphogen of Dictyostelium, induces G1 arrest and differentiation of vascular smooth muscle cells.
Circulation Research. 86(1), 68-75. (2000).

Kuwayama H, Oyama M, Kubohara Y, & Maeda M*.
A novel role of differentiation-inducing factor-1 in Dictyostelium development, assessed by the restoration of a developmental defect in a mutant lacking mitogen-activated protein kinase ERK2.
Develop. Growth & Differ. 42(5), 531-538. (2000).

[1999年]
Kubohara Y*.
Effects of differentiation-inducing factors (DIFs) of Dictyostelium discoideum on the human leukemia K562 cells: DIF-3 is the most potent anti-leukemic agent.
Eur. J. Pharmacol. 381(1), 57-62. (1999).

Kubohara Y*, & Hosaka K.
The putative morphogen, DIF-1, of Dictyostelium discoideum activates Akt/PKB in human leukemia K562 cells.
Biochem. Biophys. Res. Commun. 263(3), 790-796. (1999).

久保原禅*（和文総説：表紙写真+解説）
亜鉛が細胞分化を誘導する
蛋白質・核酸・酵素44(2), 199. (1999).

[1998年]
Kubohara Y*, Kabeya K, Matsui H, & Ishikawa K.
Effects of DIF-1, an anti-tumor agent isolated from Dictyostelium discoideum, on rat gastric mucosal RGM-1 and leptomeningeal cells.
Zool. Sci. 15(5), 713-721. (1998).

[1997年]
Kubohara Y*.
DIF-1, putative morphogen of D. discoideum, suppresses cell growth and promotes retinoic acid-induced cell differentiation in HL-60.
Biochem. Biophys. Res. Commun. 236(2), 418-422. (1997).

Asahi K*, Honma Y, Hazeki K, Sassa T, Kubohara Y, Sakurai A, & Takahashi N.
Cotylenin A, a plant-growth regulator, induces the differentiation in murine and human myeloid leukemia cells.
Biochem. Biophys. Res. Commun. 238(3), 758-763. (1997).

Kubohara Y*, & Maeda M.
Efficient induction by DIF-1 and 8-bromo cyclic AMP of prespore-to-stalk conversion in Dictyostelium discoideum.
Comp. Biochem. Physiol. 118A(3), 841-845. (1997).

久保原禅*（和文総説）
細胞性粘菌Dictyostelium discoideumの柄細胞分化誘導因子DIF-1の抗腫瘍作用
生化学 69(10), 1191-1195. (1997).

[1996年]
Saito Y, Kato M, Kubohara Y, Kobayashi I, & Tatemoto K*.
Bradykinin increases intracellular Ca2+ concentration and promotes insulin secretion in the clonal b-cell line, HIT-T15 cells.
Biochem. Biophys. Res. Commun.221(3), 577-580. (1996).

[1995年]
Kubohara Y*, Saito Y, & Tatemoto K.
Differentiation-inducing factor of D. discoideum raises intracellular calcium concentration and suppresses cell growth in rat pancreatic AR42J cells.
FEBS Lett. 359(2-3), 119-122. (1995).

Asahi K*, Sakurai A, Takahashi N, Kubohara Y, Okamoto K, & Tanaka Y.
DIF-1, morphogen of Dictyostelium discoideum, induces the erythroid differentiation in murine and human leukemia cells.
Biochem. Biophys. Res. Commun. 208(3), 1036-1039. (1995).

Kubohara Y*.
Zinc ions promote prestalk-to-stalk and prespore-to-stalk conversions in Dictyostelium discoideum.
FEMS Microbiol. Lett. 134(1), 15-18. (1995).

Kubohara Y*, Kimura C, & Tatemoto K.
Putative morphogen, DIF, of Dictyostelium discoideum induces apoptosis in rat pancreatic AR42J cells.
Develop. Growth & Differ. 37(6), 711-716. (1995).

[1994年]
Kubohara Y*, & Okamoto K.
Cytoplasmic Ca2+ and H+ concentrations determine cell fate in Dictyostelium discoideum.
FASEB Journal.8(11), 869-874. (1994).

Kubohara Y*, & Okamoto K.
Specific induction by zinc of Dictyostelium stalk cell differentiation.
Exp. Cell Res.214(1), 367-372. (1994).

[1993年]
Kubohara Y, & Okamoto K*.
DIF-resistant expression of a prespore specific gene in Dictyostelium in vitro development.
Exp. Cell Res.204(2), 382-384. (1993).

Kubohara Y*.
Purification and properties of a stalk-specific wheat germ agglutinin binding protein, Dr-st36, in the cellular slime mould, Dictyostelium rosarium.
Comp. Biochem. Physiol. 105B(1), 87-89. (1993).

Kubohara Y*, Okamoto K, Tanaka Y, Asahi K, Sakurai A, & Takahashi N.
Differanisole A, an inducer of the differentiation of Friend leukemic cells, induces stalk cell differentiation in Dictyostelium discoideum.
FEBS Lett. 322(1), 73-75. (1993).

Kubohara Y*, Maeda M, & Okamoto K.
Analysis of the maturation process of prestalk cells in Dictyostelium discoideum.
Exp. Cell Res. 207(1), 107-114. (1993).

Maeda M, & Kubohara Y*.
Protein phosphorylation during the process of prestalk-to-stalk conversion in Dictyostelium discoideum.
Develop. Growth & Differ. 35(5), 561-568. (1993).

[1992年]
Kubohara Y, & Okamoto K*.
Developmental characterization of the wheat germ agglutinin binding proteins, wst31 and wst34, enriched in prestalk and stalk cells of Dictyostelium discoideum.
Differentiation. 51(3), 163-169. (1992).

[1991年]
Kubohara Y, & Okamoto K*.
Wheat germ agglutinin binding proteins in the cellular slime moulds Dictyostelium and Polysphondylium.
J. Gen. Microbiol.(現，Microbiology-SGM) 137(7), 1571-1575. (1991).

[1990年]
Kubohara Y, & Okamoto K*.
A stalk-specific wheat germ agglutinin binding protein, wst34, in Dictyostelium discoideum can be detected with antiserum raised against Dictyostelium mucoroides stalk.
Biochem. Cell Biol. 68(4), 699-704. (1990).

[1988年]
Oyama M*, Kubohara Y, Oohata AA, & Okamoto K.
Role of cAMP and ammonia in induction and maintainance of post-aggregative differentiation in a suspension culture of Dictyostelium discoideum.
Differentiation. 38(1), 11-16. (1988).

３．特許等（筆頭発明者*）

４．競争的資金獲得

[久保原が研究代表者の研究助成]
（科研費）
令和1〜3年度 基盤研究C一般
課題：細胞性粘菌由来の生物活性物質をシードとした創薬〜新規抗がん剤と抗菌薬の開発〜

平成27〜29年度 基盤研究C一般
課題：土壌微生物「細胞性粘菌」由来の薬理活性物質DIFの研究

平成24〜26年度 基盤研究C一般
課題：土壌微生物「細胞性粘菌」由来の薬理活性物質DIFの研究

平成21〜23年度 基盤研究C一般
課題：土壌微生物「細胞性粘菌」由来の薬理活性物質DIFの研究

平成19〜20年度 基盤研究C一般
課題：土壌微生物由来の薬理活性物質DIFの作用機構解析と有用DIF誘導体の開発

平成17〜18年度 基盤研究C一般
課題：土壌微生物より単離された新規抗腫瘍因子DIFおよびその誘導体の作用機構解析

平成15〜16年度 基盤研究C一般
課題：土壌微生物より単離された新規抗腫瘍因子DIFの作用および作用機構解析

平成13〜14年度 基盤研究C一般
課題：膵幹細胞の細胞生物学

平成11〜13年度 基盤研究B展開
課題：細胞性粘菌由来の抗腫瘍因子DIFのレセプターの同定及び新規DIFアナログの開発
（採択後、久保原の英国長期出張のため辞退 → 研究代表者を変更）

平成9〜11年度 基盤研究C一般
課題：細胞性粘菌より単離された分化誘導因子DIFの抗腫瘍作用の解析

平成7年度 奨励研究A
課題：腫瘍細胞の再分化誘導因子の単離/同定と再分化機構の解析

平成6年度 奨励研究A
課題：腫瘍細胞の再分化誘導因子の単離/同定と再分化機構の解析

（JST関連助成）
平成23年度 A-STEP FSステージ 探索タイプ
課題：新規な免疫制御剤・抗炎症剤の開発

平成23年度 知財活用促進ハイウェイ「大学特許価値向上支援」
課題：細胞性粘菌由来の化合物をリードとした肥満・糖尿病治療薬の開発

平成21年度 シーズ発掘試験研究A
課題：新規免疫制御剤の開発

平成19年度 シーズ発掘試験研究
課題：新規肥満治療薬、糖尿病治療薬の開発

（民間助成）
2019年度 (公財)日本糖尿病財団 研究助成金
課題：新規肥満・糖尿病治療薬の開発とその作用機序解析

平成28年度（一財）濱口生化学振興財団 助成金
課題：新規抗菌剤の開発とその作用機序の解析

平成28年度 (一財)島原科学振興会 助成金
課題：細胞性粘菌由来の低分子物質DIFをリード化合物とした新規抗菌剤の開発

平成28年度 (公財)発酵研究所 一般研究助成金
課題：細胞性粘菌由来の低分子化合物DIFをリードとした新規抗菌剤の開発

平成19年度 (公財)武田科学振興財団「報彰基金」研究奨励
課題：土壌微生物由来の新規薬理活性物質DIFの研究

（その他）
令和3年度 群馬大学生体調節研究所「内分泌・代謝学共同研究拠点」共同研究助成
課題：新規肥満・糖尿病治療薬の開発とその作用機序解析

令和2年度 群馬大学生体調節研究所「内分泌・代謝学共同研究拠点」共同研究助成
課題：新規肥満・糖尿病治療薬の開発とその作用機序解析