English Journal of System Physiology, Graduate School of Medicine, University of Tokyo

System Physiology, Department of Biomedical Engineering,
Graduate School of Medicine,The University of Tokyo

東京大学 大学院医学系研究科 生体物理医学専攻 
医用生体工学講座 システム生理学


K. Yamamoto and J. Ando. Vascular endothelial cell membranes differentiate between stretch and shear stress through transitions in their lipid phases. Am. J. Physiol. Heart Circ. Physiol.. 309:H1178-H1185, 2015.

Y. Senju, E. Rosenbaum, C. Shah, S. Hamada-Nakahara, Y. Itoh, K. Yamamoto, K. Hanawa-Suetsugu, O. Daumke, and S. Suetsugu. Protein kinase C (PKC)-mediated phosphorylation of PACSIN2 triggers the removal of caveolae from the plasma membrane. J. Cell Sci. 128:2766-2780, 2015.


A. Kamiya, M. Shibata, and K. Yamamoto. Assessment of Myogenic Power Expenditure Due to Arterial Wall Smooth Muscle Contraction Based upon the Fractal Nature of Vascular Trees. Applied Mathematics. 5(12):1750-1762, 2014.

S. Obi, H. Masuda, H. Akimaru, T. Shizuno, K. Yamamoto, J. Ando, and T. Asahara. Dextran induces differentiation of circulating endothelial progenitor cells. Physiol. Rep. 2, e00261:1-15, 2014.

S. Obi, K. Yamamoto, and Joji Ando, Effects of shear stress on endothelial progenitor cells, J. Biomed. Nanotechnol., 10, 2586-2597, 2014


K. Yamamoto and J. Ando. Endothelial cell and model membranes respond to shear stress by rapidly decreasing the order of their lipid phases. J. Cell Sci. 126:1227-1234, 2013

J. Ando and K. Yamamoto. Flow detection and calcium signaling in vascular endothelial cells. Cardiovasc. Res. 99:260-268, 2013

Y. Abe, Y. Ozaki, J. Kasuya, K. Yamamoto, J. Ando, R. Sudo, M. Ikeda, and K. Tanishita. Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor. PLoS ONE 8 (12):1-12, 2013


S. Obi, H. Masuda,T. Shizuno, A. Sato, K. Yamamoto, J. Ando, Y. Abe, T. Asahara. Fluid shear stress induces differentiation of circulating phenotype endothelial progenitor cells. Am. J. Physiol. Cell Physiol. 303: C595-606, 2012 (Selected for Editor’s Picks)

Y. Suzuki, K. Yamamoto, J. Ando, K. Matsumoto, T. Matsuda. Arterial shear stress augments the differentiation of endothelial progenitor cells adhered to VEGF-bound surfaces. Biochem. Biophys. Res. Commun. 423: 91-97, 2012

K. Yamamoto and J. Ando, “Endothelial mechanotransduction and its role in the control of vascular functions” in Shu Chien and Shi Yongde (eds.), Recent Advances in Mechanobiology, Shanghai Science and Technological Literature Publishing House, Shanghai, pp 175-179, 2012


K. Yamamoto, K. Furuya, M. Nakamura, E. Kobatake, M. Sokabe, and J. Ando. Visualization of flow-induced ATP release and triggering of Ca2+ waves at caveolae in vascular endothelial cells. J. Cell Sci. 124: 3477-3483, 2011 (Selected for In This IssueLinkIcon)

K. Yamamoto and J. Ando. New molecular mechanisms for cardiovascular disease: Blood flow sensing mechanism in vascular endothelial cells. J. Pharmacol. Sci. 116: 323-331, 2011

A. Ishibazawa, T. Nagaoka, T. Takahashi, K. Yamamoto, J. Ando, A. Yoshida. Effects of shear stress on the gene expression of endothelial nitric oxide synthase, endothelin-1, and thronmomodulin in human retinal microvascular endothelial cells. Invest. Ophthalmol. Vis. Sci. 52: 8496-8504, 2011

T. Aoki, M. Nishimura, T. Matsuoka, K. Yamamoto, T. Furuyashiki, H. Kataoka, S. Kitaoka, R. Ishibashi, A. Ishibazawa, S. Moyamoto, R. Morishita, J. Ando, N. Hashimoto, K. Nozaki, S. Narumiya. PGE2 -EP2 receptor signaling in endothelium is activated by hemodynamic stress and induces cerebral aneurysm through an amplifying loop via NF-κB. Br. J. Pharmacol. 163: 1237-1249, 2011

J. Ando and K. Yamamoto. Effects of shear stress and stretch on endothelial cells. Antioxid. Redox Signal. 15: 1389-1403, 2011

K. Yamamoto and J. Ando, “Molecular Mechanisms Underlying Mechanosensing in Vascular Biology” in Masaki Noda (ed.), Mechanosensing Biology, Springer, Tokyo, pp 21-37, 2011


K. Yamamoto and J. Ando. Differentiation of stem/progenitor cells into vascular cells in response to fluid mechanical forces. J. Biorheol. 24: 1-10, 2010

Y-M. Chen, T. Kurokawa, T. Tominaga, K. Yasuda, Y. Osada, J-P. Gong, K. Yamamoto, J. Ando. 
Study on the sliding friction of endothelial cells cultured on hydrogel and the role of glycocalyx on friction reduction.
 Adv. Eng. Mater. 12: B628-B636, 2010

J. Ando and K. Yamamoto. “Shear-stress-sensing and response mechanisms in vascular endothelial cells” in T. Sasano and O. Suzuki (eds.), Interface Oral Health Science 2009, Springer, Tokyo, pp 3-9, 2010


T. Masumura#, K. Yamamoto#, N. Shimizu, S. Obi, and J. Ando. (# These authors equally contributed to this work.) Shear Stress Increases Expression of the Arterial Endothelial Marker EphrinB2 in Murine ES Cells via the VEGF-Notch Signaling Pathways. Arteioscler. Throm. Vasc. Biol. 29: 2125-2131, 2009

J. Ando and K. Yamamoto. Vascular Mechanobiology: Endothelial Cell Responses to Fluid Shear Stress. Circ. J. 73: 1983-1992, 2009

M. Koga, R. Sudo, Y. Abe, K. Yamamoto, J. Ando, M. Ikeda, and K. Tanishita. Contribution of rat endothelial progenitor cells on three-dimensional network formation in vitro. Tissue Eng. 15: 2727-2739, 2009

S. Obi, K. Yamamoto, N. Shimizu, S. Kumagaya, T. Masumura, T. Sokabe, T. Asahara, and J. Ando. Fluid shear stress induces arterial differentiation of endothelial progenitor cells. J. Appl. Physiol. 106: 203-211, 2009

H. Huang, Y-M. Zhou, K. Takamizawa, H. Ishibashi-Ueda, J. Ando, K. Kanda, H. Yaku, and Y. Nakayama: In vitro maturation of “Biotube” vascular grafts induced by a 2-day pulsatile flow loading. J. Biomed. Mat. Res. 91: 320-328, 2009


N. Shimizu#, K. Yamamoto#, S. Obi, S. Kumagaya, T. Masumura, Y. Shimano, K. Naruse, J. K. Yamashita, T. Igarashi, and J. Ando. (# These authors equally contributed to this work.) Cyclic strain induces mouse embryonic stem cell differentiation into vascular smooth muscle cells by activating PDGF receptor b, J. Appl. Physiol., 104: 766-772, 2008

M. Toda, K. Yamamoto, N. Shimizu, S. Obi, S. Kumagaya, T. Igarashi, A. Kamiya, and J. Ando, Differential gene responses in endothelial cells exposed to a combination of shear stress and cyclic stretch, J. Biotechnol., 133: 239-244, 2008

M. Tsuda, E. Toyomitsu, T. Komatsu, T. Masuda, E. Kunifusa, K. Nasu-Tada, S. Koizumi, K. Yamamoto, J. Ando and K. Inoue, Fibronectin/integrin system is involved in P2X4 receptor upregulation in the spinal cord and neuropathic pain after nerve injury, Glia, 56: 579-585, 2008


K. Yamamoto, N. Shimizu, S. Obi, S. Kumagaya, Y. Taketani, A. Kamiya, and J. Ando: Involvement of cell-surface ATP synthase in flow-induced ATP release by vascular endothelial cells. Am. J. Physiol. Heart Circ. Physiol. 293: H1646-H1653, 2007

S. Sugiyama, K. Yamamoto, N. Nishimura, M. Nakagawa, Y. Maruta, and J. Ando: Adequate design of customized cDNA microarray for convention multiple gene expression analysis. J. Biosci. Bioeng. 103:74-81, 2007


K. Yamamoto, T. Sokabe, T. Matsumoto, K. Yoshimura, M. Shibata, N. Ohura, T. Fukuda, T. Sato, K. Sekine, S. Kato, M. Isshiki, T. Fujita, H. Masuda, M. Kobayashi, K. Kawamura, A. Kamiya, and J. Ando. Impaired flow-dependent control of vascular tone and remodeling in P2X4-deficient mice. Nat. Med., 12: 133-137, 2006

H. Nakatsuka, T. Sokabe, K. Yamamoto, Y. Sato, K. Hatakeyama, A. Kamiya, and J. Ando. Shear stress induces hepatocyte PAI-1 gene expression through cooperative Sp1/Ets-1 activation of transcription. Am. J. Phsyiol. Gastrointest. Liver Physiol. 291:G26-G34, 2006

J.R. Mackley, J. Ando, P. Herzyk, and S.J. Winder: Phenotypic responses to mechanical stress in fibroblasts from tendon, cornea and skin. Biochem. J. 396:307-316, 2006

M. Nakamura, M. Mie, H. Funabashi, K. Yamamoto, J. Ando, and E. Kobatake. Cell-surface-localized ATP detection with immobilized firefly luciferase. Analytical Biochemistry. 352:61-67, 2006


K. Yamamoto, T. Sokabe, T. Watabe, K. Miyzono, J. K. Yamashita, S. Obi, N. Ohura, A. Matsushita, A. Kamiya, and J. Ando. Fluid shear stress induces differentiation of Flk-1-positive embryonic stem cells into vascular endothelial cells in vitro. Am. J. Physiol Heart Circ. Physiol, 288: H1915-1924, 2005. PDF

H. Huang, Y. Nakayama, K. Qin, K. Yamamoto, J. Ando, J. Yamashita, H. Itoh, K. Kanda, H. Yaku, Y. Okamoto, Y. Nemoto. Differentiation from embryonic stem cells to vascular wall cells under in vitro pulsatile flow loading. J. Artif. Organs 8:110-118. 2005

Y.M. Chen, N. Shiraishi, H. Satokawa, A. Kakugo, T. Narita, J.P. Gong, Y. Osada, K. Yamamoto, and J. Ando. Cultivation of endothelial cells on adhesive protein-free synthetic polymer gels. Biomaterials 26:4588-4596, 2005


T. Sokabe, K. Yamamoto, N. Ohura, H. Nakatsuka, K. Qin, S. Obi, A. Kamiya, and J. Ando. Differential regulation of urokinase-type plasminogen activator expression by fluid shear stress in human coronary artery endothelial cells. Am. J. Physiol. Heart Circ, Physiol 287: H2027-H2034, 2004.

E. Warabi, Y. Wada, H. Kajiwara, M. Kobayashi, N. Koshiba, T. Hisada, M. Shibata, J. Ando , M. Tsuchiya, T. Kodama, amd N. Noguch. Effect on endothelial cell gene expression of shear stress, oxygen concentration, and low-density lipoprotein as studied by a novel flow cell culture system, Free Radical Biology & Medicine, 37: 682-694, 2004

S. Ichioka, S. Kudo, K. Tokioka, M. Shibata, J. Ando, N. Sekiya, T. Nakatsuka. A symmetrical dorsal island double flap in the mouse, Eur J Plast Surg, 27: 29-32, 2004

S. Ichioka, S. Kudo, M. Shibata, J. Ando, N. Sekiya, T. Nakatsuka. Bone marrow cell implantation improves flap viability after ischemia-reperfusion injury, Ann Plast Surg, 52: 414-418, 2004


K. Yamamoto, T. Takahashi, T. Asahara, N. Ohura, T. Sokabe, A. Kamiya, and J. Ando. Proliferation, Differentiation, and Tube Formation by Endothelial Progenitor Cells in Response to Fluid Shear Stress. J. Appl. Physiol. 95: 2081-2088, 2003. PDF

N. Ohura, K. Yamamoto, S. Ichioka, T. Sokabe, H. Nakatsuka, A. Baba, M. Shibata, T. Nakatuska, K. Harii, Y. Wada, T. Kohro, T. Kodama, and J. Ando. Global Analysis of Shear Stress-Responsive Genes in Vascular Endothelial Cells. Journal of Atherosclerosis and Thrombosis. 10: 304-313, 2003.

K. Yamamoto, T. Sokabe, N. Ohura, H. Nakatsuka, A. Kamiya, and J. Ando. Endogenously released ATP mediates shear-stress-induced Ca2+ influx into pulmonary artery endothelial cells. Am. J. Physiol. 285: H793-H803, 2003. PDF

M. Iwasaka, K. Yamamoto, J. Ando, and S. Ueno. Verification of magnetic field gradient effects on medium convection and cell adhesion. J. Appl. Phys, 93: 6715-6717, 2003. PDF

D. Kaneko, T. Narita, J.P. Gong, Y. Osada, J. Ando, K. Yamamoto, S. Ohnishi, V.V. Yaminsky: Thickness decrease of a grafted polyelectrolyte membrane exposed to shear flow. J. Polym. Sci. Part B:Polym. Phys. 41:2808-2815, 2003

S. Ichioka, M. Minegishi, M. Iwasaka, M. Shibata, T. Nakatsuka, J. Ando, and S. Ueno: Skin temperature changes induced by strong static magnetic field exposure. Bioelectromagnetics 24:380-386, 2003


M. Isshiki, J. Ando, K. Yamamoto, T. Fujita, Y. Ying and R. G. W. Anderson. Site of Ca2+ wave initiation move with caveolae to the trailing edge of migrating cells. J. Cell Sci. 115: 475-484, 2002

T.C. Minh. S. Ichioka, T. Nakatsuka, J. Kawai, M. Shibata, J. Ando, and K. Harii,: Effect of hyperthermic preconditioning on the survival of ischemia-reperfused skin flaps: A new skin-flap model in the mouse. J. Reconstructive Microsurgery 18:115-120, 2002

T.C. Minh, S. Ichioka, K. Harii, M. Shibata, J. Ando, and T. Nakatsuka:Dorsal bipedicled island skin flap: a new flap model in mice. Scand J Plast Reconstr Surg Hand Surg 36: 262-267, 2002

H. Matsuoka , K. Hashimoto, S. Horikiri, M. Saito, K. Yamamoto, J. Ando. Single-cell imaging of the Ca2+ influx into bovine endothelial cells occurring in response to an alternating electric stimulus. Analytical Sciences. 18: 1205-1208, 2002

S. Ichioka, T.C. Minh, M. Shibata, T. Nakatsuka, N. Sekiya, J. Ando, and K. Harii: In vivo model for visualizing flap microcirculatin of ischemia-reperfusion. Micorsurgery 22:304-310, 2002


R. Korenaga, K. Yamamoto, N. Ohura, T. Sokabe, A. Kamiya, and J. Ando: Sp1-mediated downregulation of P2X4 receptor gene transcription in endothelial cells exposed to shear stress. Am. J. Physiol Heart Circ. Physiol. 280: H2214-H2221, 2001

M. Shibata, S. Ichioka, J. Ando, and A. Kamiya: Microvascular and interstitial PO2 measurements in rat skeletal muscle by phophorescence quenching. J. Appl. Physiol. 91: 321-327, 2001

S. Kato, J. Ando, and T. Matsuda: mRNA expression on shape-engineered endothelial cells: Adhesion molecules ICAM-1 and VCAM-1. J. Biomed. Mater. Res. 54:366-372, 2001

M. Negishi, D. Lu, Y-Q Zhang, Y. Sawada, T. Sasaki, T. Kayo, J. Ando, T. Izumi, M. Kurabayashi, I. Kojima, H. Masuda, and T. Takeuchi: Upregulatory expression of furin and Transforming growth factor-b by fluid shear stress in vascular endothelial cells. Arterioscler Thromb Vasc Biol. 21:785-790, 2001


K. Yamamoto, R. Korenaga, A. Kamiya, and J. Ando: Fluid shear stress activates Ca2+ influx into human endothelial cells via P2X4 purinoceptors. Circ. Res. 87:385-391, 2000

K. Yamamoto, R. Korenaga, A. Kamiya, Z. Qi, M. Sokabe, and J. Ando: P2X4 receptors mediate ATP-induced calcium influx in human vascular endothelial cells. Am. J. Physiol Heart Circ. Physiol. 279:H285-H292, 2000

J. Ando, H. Tsuboi, R. Korenaga, K. Takahashi and A. Kamiya. Differential display and cloning of shear stress-responsive messenger RNAs in human endothelial cells. Biochem. Biophys. Res. Commun., 225:347-351, 1996.

K. Takahashi and K. Nagai. Preparation of reactive polymeric microspheres by seeded copolymerization using a polymerizable surfactant bearing an active ester group. Polymer, 37:1257-1266, 1996

K. Takahashi, M. Suzuki, J. Kido, N. Kuramoto and K. Nagai. Alternating copolymerization of surface-active monomer having an active ester group with dialkyl fumarates. Polymer, 36:4675-4681, 1995

K. Takahashi, J. Kido, N. Kuramoto and K. Nagai. Synthesis and interfacial properties of polymerizable and nonpolymerizable surfactants having an active ester group. J. Colloid Interface Sci., 172:63-70, 1995

E. Tsuchida, H. Nishide, T. Komatsu, K. Yamamoto and K. Kobayashi. Lipid microsphere containing lipophilic heme: preparation and oxygen transportation under physiological conditions. Biochem. Biophys. Acta, 1108:253-256, 1992

M. Yuasa, K. Yamamoto, H. Nishide and E. Tsuchida. The infrared spectrum of dioxygen bound to the synthetic porphinatoion in an aqueous medium. Bull. Chem. Soc. Jpn., 61:313-315, 1988