Keita Suzuki, RPT, BS, Takashi Hiraoka, MD, PhD, Akio Tsubahara, MD, DMSc,
Tomotaka Ito, RPT, PhD, Shin-Ichi Izumi, MD, PhD, Kenji Yashima, Engineer, MS,
Nobuhisa Iwachidou, Medical Technologist, Associate Degree
Jpn J Compr Rehabil Sci 6: 56-63, 2015
Objective: The purpose of the present study was to
investigate the safety of high-frequency repetitive
peripheral magnetic stimulation (rpMS) of skeletal
muscles using histological analyses of muscles and
biochemical blood tests.
Methods: Twenty male Wistar rats were divided into
the rpMS group that received rpMS and the control
group (CON group) that did not receive rpMS.
Hematoxylin and eosin, modified Gomori trichrome,
NADH-tetrazolium reductase, acid phosphatase, and
Periodic acid-Schiff (PAS) stains were used for
histological analyses of the muscles. Creatine kinase,
aldolase, and lactate dehydrogenase (LDH) were
measured for the biochemical blood tests. Additionally,
the histological analyses of the muscles were
performed in two rats at 4 and 12 hours after completion
of rpMS application.
Results: PAS stain showed glycogen depletion in the
muscle fibers of the rpMS group. However, this
finding was not observed in the rats at 4 hours after
rpMS application. There was a significantly larger
LDH decrease in the rpMS group compared with the
CON group. No significant differences were observed
for any of the other staining methods or serum enzymes
between the two groups. There were no other abnormal
findings in the rpMS group.
Conclusion: It was established that rpMS could be
utilized safely on skeletal muscles since it did not
cause muscle damage in this study.
Key words: magnetic stimulation, skeletal muscle, safety, histological analysis of muscle, biochemical blood test