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Publications

2017

Morishita H, Kaizuka T, Hama Y, Mizushima N.

A new probe to measure autophagic flux in vitro and in vivo.

Autophagy. 2017 Jan 25. [Epub ahead of print]

Kimura H, Eguchi S, Sasaki J, Kuba K, Nakanishi H, Takasuga S, Yamazaki M, Goto A, Watanabe H, Itoh H, Imai Y, Suzuki A, Mizushima N, Sasaki T.

Vps34 regulates myofibril proteostasis to prevent hypertrophic cardiomyopathy.

JCI Insight. 2017 Jan 12;2(1):e89462. doi: 10.1172/jci.insight.89462.

Fujita N, Huang W, Lin TH, Groulx JF, Jean S, Nguyen J, Kuchitsu Y, Koyama-Honda I, Mizushima N, Fukuda M, Kiger AA.

Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy.

Elife. 2017 Jan 7;6. pii: e23367. doi: 10.7554/eLife.23367.

2016

Kaizuka T*, Morishita H* (* equally contributed), Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T, Mizushima N.

An autophagic flux probe that releases an internal control.

Mol. Cell, 2016 Nov 17;64(4):835-849.

Tsuboyama K*, Koyama-Honda I* (equal contribution), Sakamaki Y, Koike M, Morishita H, Mizushima N.

The ATG conjugation systems are important for degradation of the inner autophagosomal membrane.

Science, 2016 Nov 25;354(6315):1036-1041.

Saori R. Yoshii*, Akiko Kuma* (*equally contributed), Takumi Akashi, Taichi Hara, Atsushi Yamamoto, Yoshitaka Kurikawa, Eisuke Itakura, Satoshi Tsukamoto, Hiroshi Shitara, Yoshinobu Eishi, Noboru Mizushima.

Systemic Analysis of Atg5-Null Mice Rescued from Neonatal Lethality by Transgenic ATG5 Expression in Neurons.

Dev Cell 2016 Oct 10;39(1):116-130.

Wang C, Chen S, Yeo S, Karsli-Uzunbas G, White E, Mizushima N, Virgin HW, Guan JL.

Elevated p62/SQSTM1 determines the fate of autophagy-deficient neural stem cells by increasing superoxide.

J Cell Biol. 2016 Feb 29;212(5):545-60.

Klionsky DJ, Mizushima N, et al.

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).

Autophagy. 2016 Jan 2;12(1):1-222.

Noda NN, Mizushima N.

Atg101: Not Just an Accessory Subunit in the Autophagy-initiation Complex.

Cell Struct Funct. 2016 Feb 16;41(1):13-20.

Morishita H, Mizushima N.

Autophagy in the lens.

Exp Eye Res. 2016 Mar;144:22-8.

2015

Kaizuka T, Mizushima N.

Atg13 is essential for autophagy and cardiac development in mice.

Mol Cell Biol. 2015 Dec 7. pii: MCB.01005-15. [Epub ahead of print]

Suzuki H, Kaizuka T, Mizushima N, Noda NN.

Open and closed HORMAs regulate autophagy initiation.

Autophagy. 2015 Sep 21:0. [Epub ahead of print]

Mizushima N.

Nbr1, a Receptor for ESCRT-Dependent Endosomal Microautophagy in Fission Yeast.

Mol Cell. 2015 Sep 17;59(6):887-9.

Hieke N, Loffler AS, Kaizuka T, Berleth N, Bohler P, Driesen S, Stuhldreier F, Friesen O, Assani K, Schmitz K, Peter C, Diedrich B, Dengjel J, Holland P, Simonsen A, Wesselborg S, Mizushima N, Stork B.

Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells.

Autophagy. 2015 Jul 25:0. [Epub ahead of print]

Suzuki H*, Kaizuka T* (*equally contributed), Mizushima N#, Noda NN#(#co-corresponding authors).

Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation.

Nat Struct Mol Biol. 2015 Jun 1. doi: 10.1038/nsmb.3036.

Zhao YG, Sun L, Miao G, Ji C, Zhao H, Sun H, Miao L, Yoshii SR, Mizushima N, Wang X, Zhang H.

The autophagy gene Wdr45/Wipi4 regulates learning and memory function and axonal homeostasis.

Autophagy. 2015 May 22:0. [Epub ahead of print]

Schlie K, Westerback A, DeVorkin L, Hughson LR, Brandon JM, MacPherson S, Gadawski I, Townsend KN, Poon VI, Elrick MA, Cote HC, Abraham N, Wherry EJ, Mizushima N, Lum JJ.

Survival of Effector CD8+ T Cells during Influenza Infection Is Dependent on Autophagy.

J Immunol. 2015 May 1;194(9):4277-86.

Yoshii SR, Mizushima N.

Autophagy machinery in the context of mammalian mitophagy.

Methods. 2015 Mar;75:13-8. doi: 10.1016/j.ymeth.2014.11.021.

Jiang P, Mizushima N.

LC3- and p62-based biochemical methods for the analysis of autophagy progression in mammalian cells.

Biochim Biophys Acta. 2015 Jan 26. pii: S0167-4889(15)00022-1.

Ishihara T, Ban-Ishihara R, Maeda M, Matsunaga Y, Ichimura A, Kyogoku S, Aoki H, Katada S, Nakada K, Nomura M, Mizushima N, Mihara K, Ishihara N.

Dynamics of mitochondrial DNA nucleoids regulated by mitochondrial fission is essential for maintenance of homogeneously active mitochondria during neonatal heart development.

Mol Cell Biol. 2015 Jan;35(1):211-23.

2014

Mizushima N.

Sugar modification inhibits autophagosome-lysosome fusion.

Nat Cell Biol. 2014 Dec;16(12):1132-3. doi: 10.1038/ncb3078.

Kishi-Itakura C, Koyama-Honda I, Itakura E, Mizushima N.

Ultrastructural analysis of autophagosome organization using mammalian autophagy-deficient cells.

J Cell Sci. 2014 Jul 22. pii: jcs.156034.

Watanabe-Asano T, Kuma A, Mizushima N.

   Cycloheximide inhibits starvation-induced autophagy through mTORC1 activation.

   Biochem Biophys Res Commun.

       2014 Mar 7;445(2):334-9. doi: 10.1016/j.bbrc.2014.01.180. Epub 2014 Feb 10.

Jiang P, Nishimura T, Sakamaki Y, Itakura E, Hatta T, Natsume T, Mizushima N
The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.
Mol Biol Cell. 2014 Feb 19. [Epub ahead of print]
 
Sahani MH, Itakura E, Mizushima N.
Expression of the autophagy substrate SQSTM1/p62 is restored during prolonged starvation depending on transcriptional upregulation and autophagy-derived amino acids.
Autophagy. 2014 Jan 3;10(3). [Epub ahead of print]
Shen HM, Mizushima N.
At the end of the autophagic road: an emerging understanding of lysosomal functions in autophagy.
Trends Biochem Sci. 2014 Feb;39(2):61-71.
Jiang P, Mizushima N.
Autophagy and human diseases.
Cell Res. 2014 Jan;24(1):69-79. doi: 10.1038/cr.2013.161.
 

2013

Koyama-Honda I, Itakura E, Fujiwara TK, Mizushima N.
Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site.
Autophagy 2013 Jul 10;9(10). [Epub ahead of print]
Naito T, Kuma A, Mizushima N
Differential contribution of insulin and amino acids to the mTORC1-autophagy pathway in the liver and muscle.
J Biol Chem. 2013 Jul 19;288(29):21074-81
Morishita H, Eguchi S, Kimura H, Sasaki J, Sakamaki Y, Robinson ML, Sasaki T, Mizushima N
Deletion of autophagy-related 5 (Atg5) and Pik3c3 in the lens causes cataract independent of programmed organelle degradation.
J Biol Chem. 2013 Apr 19;288(16):11436-47.
Saitsu H*, Nishimura T*, Muramatsu K* (*equally contributed), Kodera H, Kumada S, Sugai K, Kasai-Yoshida E, Sawaura N, Nishida H, Hoshino A, Ryujin F, Yoshioka S, Nishiyama K, Kondo Y, Tsurusaki Y, Nakashima M, Miyake N, Arakawa H, Kato M, Mizushima N, Matsumoto N
De novo mutations in the autophagy gene WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood.
Nature Genet. 2013 doi:10.1038/ng.2562
Nishimura T, Kaizuka T, Cadwell K, Sahani MH, Saitoh T, Akira S, Virgin HW, Mizushima N
FIP200 regulates targeting of Atg16L1 to the isolation membrane.
EMBO Rep. 2013 Feb 8. doi: 10.1038/embor.2013.6. [Epub ahead of print]
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Karanasios E, Stapleton E, Manifava M, Kaizuka T, Mizushima N, Walker SA, Ktistakis NT.
Dynamic association of the ULK1 complex with omegasomes during autophagy induction.
J Cell Sci. 2013 Sep 6. [Epub ahead of print]
Seino J, Wang L, Harada Y, Huang C, Ishii K, Mizushima N, Suzuki T.
Basal autophagy is required for the efficient catabolism of sialyl oligosaccharides.
J Biol Chem. 2013 Jul 23. [Epub ahead of print]
 

2012

Itakura E, Kishi-Itakura C, Mizushima N
The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.
Cell 2012 Dec 7; 151 (6): 1256-1269
Quy PN, Kuma A, Pierre P and Mizushima N
Proteasome-dependent activation of mammalian target of rapamycin complex 1 (mTORC1) is essential for autophagy suppression and muscle remodeling following denervation.
J Biol Chem. 2012 Dec 3. [Epub ahead of print]
Kitamura K, Kishi-Itakura C, Tsuboi T, Sato S, Kita K, Ohta N, Mizushima N.
Autophagy-Related Atg8 Localizes to the Apicoplast of the Human Malaria Parasite Plasmodium falciparum.
PLoS One. 2012;7(8):e42977. Epub 2012 Aug 10.
Itakura E, Kishi-Itakura C, Koyama-Honda I, Mizushima N.
Structures containing Atg9A and the ULK1 complex independently target depolarized mitochondria at initial stages of Parkin-mediated mitophagy.
J Cell Sci. 2012 Jan 24. [Epub ahead of print]
Velikkakath AK, Nishimura T, Oita E, Ishihara N, Mizushima N.
Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets.
Mol Biol Cell. 2012 Jan 4. [Epub ahead of print]
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Lin TC, Chen YR, Kensicki E, Li AY, Kong M, Li Y, Mohney RP, Shen HM, Stiles B, Mizushima N, Lin LI, Ann DK.
Autophagy: Resetting glutamine-dependent metabolism and oxygen consumption.
Autophagy. 2012 Oct 1;8(10).
Liu S, Hartleben B, Kretz O, Wiech T, Igarashi P, Mizushima N, Walz G, Huber TB.
Autophagy plays a critical role in kidney tubule maintenance, aging and ischemia-reperfusion injury.
Autophagy. 2012 May 1;8(5). [Epub ahead of print]
Onoue K, Jofuku A, Ban-Ishihara R, Ishihara T, Maeda M, Koshiba T, Itoh T, Fukuda M, Otera H, Oka T, Takano H, Mizushima N, Mihara K, Ishihara N.
Fis1 acts as mitochondrial recruitment factor for TBC1D15 that involved in regulation of mitochondrial morphology.
J Cell Sci. 2012 Oct 17. [Epub ahead of print]
Imaizumi Y, Okada Y, Akamatsu W, Koike M, Kuzumaki N, Hayakawa H, Nihira T, Kobayashi T, Ohyama M, Sato S, Takanashi M, Funayama M, Hirayama A, Soga T, Hishiki T, Suematsu M, Yagi T, Ito D, Kosakai A, Hayashi K, Shouji M, Nakanishi A, Suzuki N, Mizuno Y, Mizushima N, Amagai M, Uchiyama Y, Mochizuki H, Hattori N, Okano H.
Mitochondrial dysfunction associated with increased oxidative stress and α-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue.
Mol Brain. 2012 Oct 6;5:35. doi: 10.1186/1756-6606-5-35.

2011

Takamura A, Komatsu M, Hara T, Sakamoto A, Kishi C, Waguri S, Eishi Y, Hino O, Tanaka K, Mizushima N
Autophagy-deficient mice develop multiple liver tumors
Genes Dev. 2011 Apr 15;25(8):795-800.
Yoshii SR, Kishi C, Ishihara N, Mizushima N.
Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane.
J Biol Chem. 2011 Jun 3;286(22):19630-40. Epub 2011 Mar 18.
Itakura E, Mizushima N.
p62 targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding.
J Cell Biol. 192: 17-27 (2011).
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Klionsky DJ, Baehrecke EH, Brumell JH, Chu CT, Codogno P, Cuervo AM, Debnath J, Deretic V, Elazar Z, Eskelinen EL, Finkbeiner S, Fueyo-Margareto J, Gewirtz D, Jaattela M, Kroemer G, Levine B, Melia TJ, Mizushima N, Rubinsztein DC, Simonsen A, Thorburn A, Thumm M, Tooze SA.
A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.
Autophagy. 2011 Nov 1;7(11):1273-94. Epub 2011 Nov 1.
Katayama H, Kogure T, Mizushima N, Yoshimori T, Miyawaki A.
A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.
Chem Biol. 2011 Aug 26;18(8):1042-52.
Asano T, Komatsu M, Yamaguchi-Iwai Y, Ishikawa F, Mizushima N, Iwai K.
Distinct mechanisms of ferritin delivery to lysosomes in iron-depleted and iron-replete cells.
Mol Cell Biol. 2011 May;31(10):2040-52. Epub 2011 Mar 28.

2010

Itakura E, Mizushima N.
Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins.
Autophagy. 2010 Aug 16;6(6):764-76.
Kaizuka T, Hara T, Oshiro N, Kikkawa U, Yonezawa K, Takehana K, Iemura SI, Natsume T, Mizushima N.
Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly.
J Biol Chem. 2010 Jun 25;285(26):20109-16. Epub 2010 Apr 28.
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Riley BE, Kaiser SE, Shaler TA, Ng AC, Hara T, Hipp MS, Lage K, Xavier RJ, Ryu KY, Taguchi K, Yamamoto M, Tanaka K, Mizushima N, Komatsu M, Kopito RR.
Ubiquitin accumulation in autophagy-deficient mice is dependent on the Nrf2-mediated stress response pathway: a potential role for protein aggregation in autophagic substrate selection.
J Cell Biol. 2010 Nov 1;191(3):537-52.
 
Taneike M, Yamaguchi O, Nakai A, Hikoso S, Takeda T, Mizote I, Oka T, Tamai T, Oyabu J, Murakawa T, Nishida K, Shimizu T, Hori M, Komuro I, Shirasawa T, Mizushima N, Otsu K.
Inhibition of autophagy in the heart induces age-related cardiomyopathy..
Autophagy. 2010 Jul 1;6(5).
Hartleben B, Godel M, Meyer-Schwesinger C, Liu S, Ulrich T, Kobler S, Wiech T, Grahammer F, Arnold SJ, Lindenmeyer MT, Cohen CD, Pavenstadt H, Kerjaschki D, Mizushima N, Shaw AS, Walz G, Huber TB.
Autophagy influences glomerular disease susceptibility and maintains podocyte homeostasis in aging mice.
J Clin Invest. 2010 Apr 1;120(4):1084-96.
Lee HK, Mattei LM, Steinberg BE, Alberts P, Lee YH, Chervonsky A, Mizushima N, Grinstein S, Iwasaki A.
In Vivo Requirement for Atg5 in Antigen Presentation by Dendritic Cells.
Immunity. 2010 Feb 26;32(2):227-239.
Nishiyama J, Matsuda K, Kakegawa W, Yamada N, Motohashi J, Mizushima N, Yuzaki M.
Reevaluation of Neurodegeneration in lurcher Mice: Constitutive Ion Fluxes Cause Cell Death with, Not by, Autophagy.
J Neurosci. 2010 Feb 10;30(6):2177-87.
Katsumata K, Nishiyama J, Inoue T, Mizushima N, Takeda J, Yuzaki M.
Dynein- and activity-dependent retrograde transport of autophagosomes in neuronal axons.
Autophagy. 2010 Apr;6(3):378-85. Epub 2010 Apr 20.

2009

Hosokawa, N., Sasaki, T., Iemura, S., Natsume, T., Hara, T., Mizushima, N.
Atg101, a novel mammalian autophagy protein interacting with Atg13.
Autophagy 5:973-9 (2009)
Hosokawa, N., Hara, T., Kaizuka, T., Kishi, C., Takamura, A., Miura, Y., Iemura, S., Natsume, T., Takehana, K., Yamada, N., Guan, J.L., Oshiro, N., Mizushima, N.
Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy.
Mol. Biol. Cell 20: 1981-1991 (2009)
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Inoue K, Kuwana H, Shimamura Y, Ogata K, Taniguchi Y, Kagawa T, Horino T, Takao T, Morita T, Sasaki S, Mizushima N, Terada Y.
Cisplatin-induced macroautophagy occurs prior to apoptosis in proximal tubules in vivo.
Clin Exp Nephrol. 2009 Dec 15.
Satoo, K., Noda, N.N., Kumeta, H., Fujioka, Y., Mizushima, N., Ohsumi, Y., Inagaki, F.
The structure of Atg4B-LC3 complex reveals the mechanism of LC3 processing and delipidation during autophagy.
EMBO J. 28: 1341-50 (2009)
Stephenson, L.M., Miller, B.C., Ng, A., Eisenberg, J., Zhao, Z., Cadwell, K., Graham, D.B., Mizushima, N., Xavier, R., Virgin, H.W., Swat, W.
Identification of Atg5-dependent transcriptional changes and increases in mitochondrial mass in Atg5-deficient T lymphocytes.
Autophagy 5: 625-35 (2009)

2008

Itakura, E., Kishi, C., Inoue, K., Mizushima, N.
Beclin 1 Forms Two Distinct Phosphatidylinositol 3-Kinase Complexes with Mammalian Atg14 and UVRAG.
Mol. Biol. Cell 19: 5360-5372 (2008)
Tsukamoto, S., Kuma, A., Murakami, M., Kishi, C., Yamamoto, A., Mizushima, N.
Autophagy is essential for preimplantation development of mouse embryos.
Science 321: 117-120 (2008)
Hara, T., Takamura, A., Kishi, C., Iemura, S., Natsume, T., Guan, J.L., Mizushima, N.
FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells.
J. Cell Biol. 181:497-510 (2008)
Kuma, A., Mizushima, N.
Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice
Autophagy 4:61-62 (2008)
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Zhao, Z., Fux, B., Goodwin, M., Dunay, I.R., Strong, D., Miller, B.C., Cadwell, K., Delgado, M.A., Ponpuak, M., Green, K.G., Schmidt, R.E., Mizushima, N., Deretic, V., Sibley, L.D., Virgin, IV H.W.
Autophagosome-independent essential function for the autophagy protein Atg5 in cellular immunity to intracellular pathogens.
Cell Host Microbe. 4: 458-469 (2008)
Cadwell, K., Liu, J.Y., Brown, S.L., Miyoshi, H., Loh, J., Lennerz, J.K., Kishi, C., Kc, W., Carrero, J.A., Hunt, S., Stone, C.D., Brunt, E.M., Xavier, R.J., Sleckman, B.P., Li, E., Mizushima, N., Stappenbeck, T.S., Virgin, IV H.W.
A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells.
Nature. 456: 259-263 (2008)
Ohne, Y., Takahara, T., Hatakeyama, R., Matsuzaki, T., Noda, M., Mizushima, N., Maeda, T.
Isolation of hyperactive mutants of mammalian target of rapamycin.
J. Biol. Chem. 283: 31861-31870 (2008)
Gozuacik, D., Bialik, S., Raveh, T., Mitou, G., Shohat, G., Sabanay, H., Mizushima, N., Yoshimori, T., Kimchi, A.
DAP-kinase is a mediator of endoplasmic reticulum stress-induced caspase activation and autophagic cell death.
Cell Death Differ. 15: 1875-1886 (2008)
Raben, N., Hill, V., Shea, L., Takikita, S., Baum, R., Mizushima, N., Ralston, E., Plotz, P.
Suppression of autophagy in skeletal muscle uncovers the accumulation of ubiquitinated proteins and their potential role in muscle damage in Pompe disease.
Hum Mol Genet. 17: 3897-3908 (2008)
Sou, Y.S., Waguri, S., Iwata, J.I., Ueno, T., Fujimura, T., Hara, T., Sawada, N., Yamada, A., Mizushima, N., Uchiyama, Y., Kominami, E., Tanaka, K., Komatsu, M.
The Atg8 Conjugation System Is Indispensable for Proper Development of Autophagic Isolation Membranes in Mice.
Mol Biol Cell. 19: 4762-4775 (2008)
Nedjic, J., Aichinger, M., Emmerich, J., Mizushima, N., Klein, L.
Macroautophagy in thymic epithelial cells shapes the T cell repertoire and is essential for self-tolerance.
Nature 455: 396-400 (2008)
Hashimoto, D., Ohmuraya, M., Hirota, M., Yamamoto, A., Suyama, K., Ida, S., Okumura, Y., Takahashi, E., Kido, H., Araki, K., Baba, H., Mizushima, N., Yamamura, K.,
Involvement of autophagy in trypsinogen activation within the pancreatic acinar cells.
J. Cell Biol. 181: 1065-1072 (2008)
Kaushik, S., Massey, A. C., Mizushima, N., Cuervo, A. M.
Constitutive activation of chaperone-mediated autophagy in cells with impaired macroautophagy.
Mol. Biol. Cell 19:2179-2192 (2008)
Talloczy, Z., Martinez, J., Joset, D., Ray, Y., Gacser, A., Toussi, S., Mizushima, N., Nosanchuk, J., Goldstein, H., Loike, J., Sulzer, D., Santambrogio, L.
Methamphetamine Inhibits Antigen Processing, Presentation, and Phagocytosis.
PLoS Pathog. 4:e28 (2008)
Katayama, H., Yamamoto, A., Mizushima, N., Yoshimori, T., Miyawaki, A.
GFP-like Proteins Stably Accumulate in Lysosomes.
Cell Struct. Funct. 33:1-12 (2008)
King, M.A., Hands, S., Hafiz, F., Mizushima, N., Tolkovsky, A.M., Wyttenbach, A.
Rapamycin inhibits polyglutamine aggregation independently of autophagy by reducing protein synthesis.
Mol. Pharmacol. 73:1052-63 (2008)
Miller, B.C., Zhao, Z., Stephenson, L.M., Cadwell, K., Pua, H.H., Lee, H.K., Mizushima, N., Iwasaki, A., He, Y.W., Swat, W., Virgin, H.W.
The autophagy gene ATG5 plays an essential role in B lymphocyte development.
Autophagy 4:309-14 (2008)

[ to the TOP]

2007

Kuma, A., Matsui, M. and Mizushima, N.
LC3, an autophagosome marker, can be incorporated into protein aggregates independent of autophagy: Caution in the interpretation of LC3 localization.
Autophagy 3:323-328 (2007)
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Nishiyama J, Miura E, Mizushima N, Watanabe M, Yuzaki M.
Aberrant membranes and double-membrane structures accumulate in the axons of Atg5-null Purkinje cells before neuronal death.
Autophagy 3:591-596 (2007)
Komatsu, M., Waguri, S., Koike, M., Sou, Y.S., Ueno, T., Hara, T., Mizushima, N., Iwata, J.I., Ezaki, J., Murata, S., Hamazaki, J., Nishito, Y., Iemura, S.I., Natsume, T., Yanagawa, T., Uwayama, J., Warabi, E., Yoshida, H., Ishii, T., Kobayashi, A., Yamamoto, M., Yue, Z., Uchiyama, Y., Kominami, E., Tanaka, K.
Homeostatic Levels of p62 Control Cytoplasmic Inclusion Body Formation in Autophagy-Deficient Mice.
Cell 131:1149-1163 (2007)
Alexander, D.E., Ward, S.L., Mizushima, N., Levine, B., Leib, D.A.
An analysis of the role of autophagy in replication of herpes simplex virus in cell culture.
J. Virol. 81:12128-12134 (2007)
Zhao, Z., Thackray, L.B., Miller, B.C., Lynn, T.M., Becker, M.M., Ward, E., Mizushima, N., Denison, M.R., Virgin, H.W. 4th.
Coronavirus Replication Does Not Require the Autophagy Gene ATG5.
Autophagy 3:581-585 (2007)
Nakai, A., Yamaguchi, O., Takeda, T., Higuchi, Y., Hikoso, S., Taniike, M., Omiya, S., Mizote, I., Matsumura, Y., Asahi, M., Nishida, K., Hori, M., Mizushima, N., Otsu, K.
The role of autophagy in cardiomyocytes in the basal state and in response to hemodynamic stress.
Nat. Med. 13:619-24 (2007)
Marino, G., Salvador-Montoliu, N., Fueyo, A., Knecht, E., Mizushima, N., Lopez-Otin, C.
Tissue-specific autophagy alterations and increased tumorigenesis in mice deficient in ATG4C/autophagin-3.
J. Biol. Chem. 282:18573-18583 (2007)
Lee, H.K., Lund, J.M., Ramanathan, B., Mizushima, N., Iwasaki, A.
Autophagy-dependent viral recognition by plasmacytoid dendritic cell.
Science 315:1398-1401 (2007)
Satoo, K., Suzuki, N.N., Fujioka, Y., Mizushima, N., Ohsumi, Y., Inagaki, F.
Crystallization and preliminary crystallographic analysis of human Atg4B?LC3 complex. Acta Crystallograph.
Sect. F Struct. Biol. Cryst. Commun. 63:99-102 (2007)
Pua, H.H., Dzhagalov, I., Chuck, M., Mizushima, N., He, Y-W.
A critical role for the autophagy gene Atg5 in T cell survival and proliferation.
J. Exp. Med. 204:25-31 (2007)

2006

Hosokawa, N., Hara, Y., Mizushima, N.
Generation of cell lines with tetracycline-regulated autophagy and a role for autophagy in controlling cell size.
FEBS Lett. 580, 2623-2629 (2006).
Hara, T., Nakamura, K., Matsui, M., Yamamoto, A., Nakahara, Y., Suzuki-Migishima, R. Yokoyama, M., Mishima, K., Saito, I., Okano, H., Mizushima, N.
Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice.
Nature 441, 885-889 (2006).
Matsui, M., Yamamoto, A., Kuma, A., Ohsumi, Y., Mizushima, N.
Organelle degradation during the lens and erythroid differentiation is independent of autophagy.
Biochem. Biophys. Res. Commun. 339, 485-489 (2006).
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Adhami, F., Liao, G., Morozov, Y. M., Schloemer, A., Schmithorst, V. J., Lorenz, J. N., Dunn, R. S., Vorhees, C. V., Wills-Karp, M., Degen, J. L., Davis, R. J., Mizushima, N., Rakic, P., Dardzinski, B. J., Holland, S. K., Sharp, F. R., Kuan, C.-Y.
Cerebral ischemia-hypoxia induces intravascular coagulation and autophagy.
Am. J. Pathol. 169, 566-583 (2006)
Wang, Q.J., Ding, Y., Zhong, Y., Kohtz, S., Mizushima, N., Cristea, I.M., Rout, M.P., Chait, B.T., Heintz, N. and Yue, Z.
Induction of autophagy in axonal dystrophy and degeneration.
J. Neurosci. 26, 8057-8068 (2006).
Szeto, J., Kaniuk, N.A., Canadien, V., Nisman, R., Mizushima, N., Yoshimori, T., Bazett-Jones, D.P., Brumell, J.H. ALIS are stress-induced protein storage compartments for substrates of the proteasome and autophagy.
Autophagy 2, 189-199 (2006).
Shibata. M., Lu, T., Furuya, T., Degterev, A., Mizushima, N., Yoshimori, T., Macdonald, M., Yankner, B., Yuan, J. Regulation of intracellular accumulation of mutant Huntingtin by Beclin 1.
J. Biol. Chem.281, 14474-14485 (2006)
Zhang, H., Monken, C.E., Zhang, Y., Lenard, J., Mizushima, N., Lattime, E.C., Jin, S.
Cellular autophagy machinery is not required for vaccinia virus replication and maturation.
Autophagy 2, 91-95 (2006).
Kamimoto, T., Shoji, S., Hidvegi, T., Mizushima, N., Umebayashi, K., Perlmutter, D.H., Yoshimori, T.
Intracellular inclusions containing mutant alpha 1-antitrypsin Z are propagated in the absence of autophagic activity.
J. Biol. Chem. 281, 4467-4476 (2006).

2005

Koike, M., Shibata, M., Waguri, S., Yoshimura, K., Tanida, I., Kominami, E., Gotow, T., Peters, C., von Figura, K., Mizushima, N., Saftig, P., Uchiyama, Y.
Participation of autophagy in storage of lysosomes in neurons from mouse models of neuronal ceroid-lipofuscinoses (batten disease).
Am. J. Pathol. 167, 1713-28 (2005).
Hara, T., Kamura, T., Kotoshiba, S., Takahashi, H., Fujiwara, K., Onoyama, I., Shirakawa, M., Mizushima, N., Nakayama, K.I.
Role of the UBL-UBA protein KPC2 in degradation of p27 at G1 phase of the cell cycle.
Mol. Cell. Biol.25, 9292-9303 (2005).
Sugawara, K., Suzuki, N.N., Fujioka, Y., Mizushima, N., Ohsumi, Y., Inagaki, F.
Structural basis for the specificity and catalysis of human Atg4B responsible for mammalian autophagy.
J Biol Chem. 280, 40058-40065 (2005).
Pattingre, S., Tassa, A., Qu, X., Garuti, R., Huan Liang, X., Mizushima, N., Packer, M., Schneider, M.D., Levine, B.
Bcl-2 Antiapoptotic Proteins Inhibit Beclin 1-Dependent Autophagy.
Cell 122, 927-939 (2005).
Ohmuraya, M., Hirota, M., Araki, M., Mizushima, N., Matsui, M., Mizumoto, T., Haruna, K., Kume, S., Takeya, M., Ogawa, M., Araki, K., Yamamura, K.
Autophagic cell death of pancreatic acinar cells in serine protease inhibitor kazal type 3-deficient mice.
Gastroenterology 129, 696-705 (2005).
Degterev, A., Huang, Z., Boyce, M., Li, Y., Jagtap, P., Mizushima, N., Cuny, G.D., Mitchison, T.J., Moskowitz, M.A., Yuan, J.
Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury.
Nat. Chem. Biol. 1, 112-119 (2005)
Komatsu, M., Waguri, S., Ueno, T., Iwata, J., Murata, S., Tanida, I., Ezaki, J., Mizushima, N., Ohsumi, Y., Uchiyama, Y., Kominami, E., Tanaka, K., Chiba, T.
Impairment of starvation-induced and contitutive autophagy in Atg7-deficient mice.
J. Cell Biol. 169, 425-434 (2005)
Pyo, J.O., Jang, M.H., Kwon, Y.K., Lee, H.J., Jun, J.I., Woo, H.N., Cho, D.H., Choi, B., Lee, H., Kim, J.H., Mizushima, N., Oshumi, Y., Jung, Y.K.
Essential roles of Atg5 and FADD in autophagic cell death: Dissection of autophagic cell death into vacuole formation and cell death.
J Biol Chem. 280, 20722-20729 (2005)
Bampton, E.T.W., Goemans, C.G., Niranjan, D., Mizushima, N., Tolkovsky, A.M.
The dynamics of autophagy visualised in live cells: from autophagosome formation to fusion with endo/lysosomes.
Autophagy 1, 23-36 (2005)
Ogawa, M., Yoshimori, T., Suzuki, T., Sagara, H., Mizushima, N., Sasakawa, C.
Escape of Intracellular Shigella from Autophagy.
Science 307, 727-731 (2005)

[to the TOP]

2004

Kuma, A., Hatano, M., Matsui, M., Yamamoto, A., Nakaya, H., Yoshimori, T., Ohsumi, Y., Tokuhisa, T., Mizushima, N.
The role of autophagy during the early neonatal starvation period.
Nature 432, 1032-1036 (2004).
Mizushima, N., Yamamoto, A., Matsui, M., Yoshimori, T. and Ohsumi, Y.
In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker.
Mol. Biol. Cell 15, 1101-1111 (2004).
------------------------------------------------
Shimizu, S., Kanaseki, T., Mizushima, N., Mizuta, T., Arakawa-Kobayashi, S., Thompson, C.B., Korsmeyer, S.J. Tsujimoto, Y.
A role of Bcl-2 family of proteins in non-apoptotic programmed cell death dependent on autophagy genes.
Nat. Cell Biol. 12,1221-1228 (2004)
Nakagawa, I., Amano, A., Mizushima, N., Yamamoto, A., Yamaguchi, H., Kamimoto, T., Nara, A., Funao, J., Nakata, M., Tsuda, K., Hamada, S., Yoshimori, T.
Autophagy defends cells against invading group A Streptococcus.
Science 306, 1037-1040 (2004).
Prentice,E., Jerome, WG, Yoshimori, T., Mizushima, N., Denison, MR.
Coronavirus Replication Complex Formation Utilizes Components of Cellular Autophagy
J. Biol. Chem. 279, 10136-10141 (2004).
Kabeya, Y., Mizushima, N., Yamamoto, A., Oshitani-Okamoto, S., Ohsumi, Y. and Yoshimori, T.
LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation.
J. Cell Sci. 117, 2805-2812 (2004).
Birkeland, H., Simonsen, A., Gillooly, D.J., Mizushima, N., Kuma, A., Yoshimori, T., Slagsvold, T., Brech, A., Stenmark, H.
Alfy, a novel FYVE domain-containing protein associated with protein granules and autophagic membranes.
J. Cell Sci. 117, 4239-4251 (2004).
Shitara, H., Sato, A., Hayashi, J., Mizushima, N., Yonekawa, H., Taya, C.
Simple method of zygosity identification in transgenic mice by real-time quantitative PCR.
Transgenic Research 13, 191-194 (2004).
Sugawara K, Suzuki NN, Fujioka Y, Mizushima N, Ohsumi Y, Inagaki F.
The crystal structure of microtubule-associated protein light chain 3, a mammalian homologue of Saccharomyces cerevisiae Atg8.
Genes Cells 9, 611-618 (2004).

[ to the TOP]

-2003 (selected)

Mizushima, N., Kuma, A., Kobayashi, Y., Yamamoto, A., Matsubae, M., Takao, T., Natsume, T., Ohsumi, Y. and Yoshimori, T.
Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate
J. Cell Sci. 116, 1679-1688. (2003)
Qu X, Yu J, Bhagat G, Furuya N, Hibshoosh H, Troxel A, Rosen J, Eskelinen EL, Mizushima N, Ohsumi Y, Cattoretti G, Levine B.
Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene.
J. Clin. Invest. 112, 1809-1820. (2003)
Ohashi, M., Mizushima, N., Kabeya, Y. and Yoshimori, T.
Localization of mammalian NAD(P)H steroid dehydrogenase-like protein on lipid droplets
J. Biol. Chem. 278, 36819-36829.(2003)
Mizushima, N., Yoshimori, T. and Ohsumi, Y.
Mouse Apg10 as an Apg12 conjugating enzyme: Analysis by the conjugation-mediated yeast two-hybrid method
FEBS Lett. 532, 450-454. (2002)
Kuma, A., Mizushima, N., Ishihara, N. and Ohsumi, Y.
Formation of the ~350 kD Apg12-Apg5-Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast
J. Biol. Chem. 277, 18619-18625. (2002)
Suzuki, K., Kirisako, T., Kamada, Y., Mizushima, N., Noda, T. and Ohsumi, Y.
The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation
EMBO J. 20, 5971-5981. (2001)
Mizushima, N., Yamamoto, A., Hatano, M., Kobayashi, Y., Kabeya, Y., Suzuki, K., Tokuhisa, T., Ohsumi, Y. and Yoshimori, T.
Dissection of Autophagosome Formation using Apg5-Deficient Mouse Embryonic Stem Cells
J. Cell Biol. 152, 657-667. (2001)
Yoshimori, T., Yamagata, F., Yamamoto, A., Mizushima, N., Kabeya, Y., Nara, A., Miwako, I., Ohashi, M., Ohsumi, M. and Ohsumi, Y.
The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells
Mol. Biol. Cell 11, 747-63. (2000)
Kirisako, T., Ichimura, Y., Okada, H., Kabeya, Y., Mizushima, N., Yoshimori, T., Ohsumi, M., Takao, T., Noda, T. and Ohsumi, Y.
The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway
J. Cell Biol. 151, 263-275. (2000)
Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y. and Yoshimori, T.
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
EMBO J. 19, 5720-5728. (2000)
Ichimura, Y., Kirisako, T., Takao, T., Satomi, Y., Shimonishi, Y., Ishihara, N., Mizushima, N., Tanida, I., Kominami, E., Ohsumi, M., Noda, T. and Ohsumi, Y.
A ubiquitin-like system mediates protein lipidation
Nature 408, 488-492. (2000)
George, M. D., Baba, M., Scott, S. V., Mizushima, N., Garrison, B. S., Ohsumi, Y. and Klionsky, D. J.
Apg5p functions in the sequestration step in the cytoplasm-to-vacuole targeting and macroautophagy pathways
Mol. Biol. Cell 11, 969-82. (2000)
Furukawa, K., Mizushima, N., Noda, T. and Ohsumi, Y.
A Protein Conjugation System in Yeast with Homology to Biosynthetic Enzyme Reaction of Prokaryotes
J. Biol. Chem 275, 7462-7465. (2000)
Tanida, I., Mizushima, N., Kiyooka, M., Ohsumi, M., Ueno, T., Ohsumi, Y. and Kominami, E.
Apg7p/Cvt2p: a novel protein activating enzyme essential for autophagy
Mol. Biol. Cell 10, 1367-1379. (1999)
Shintani, T., Mizushima, N., Ogawa, Y., Matsuura, A., Noda, T. and Ohsumi, Y.
Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast
EMBO J. 18, 5234-5241. (1999)
Mizushima, N., Noda, T. and Ohsumi, Y.
Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway
EMBO J. 18, 3888-3896. (1999)
Mizushima, N., Sugita, H., Yoshimori, T. and Ohsumi, Y.
A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy
J. Biol. Chem. 273, 33889-33892. (1998)
Mizushima, N., Noda, T., Yoshimori, T., Tanaka, Y., Ishii, T., George, M. D., Klionsky, D. J., Ohsumi, M. and Ohsumi, Y.
A protein conjugation system essential for autophagy
Nature 395, 395-398. (1998)

[ to the TOP]

Review Article

Shpilka T, Mizushima N, Elazar Z.
Ubiquitin-like proteins and autophagy at a glance.
J Cell Sci. 2012 May 15;125(Pt 10):2343-8. doi: 10.1242/jcs.093757.
Klionsky DJ et al.
Guidelines for the use and interpretation of assays for monitoring autophagy.
Autophagy. 2012 Apr;8(4):445-544.
Mizushima N, Komatsu M.
Autophagy: renovation of cells and tissues.
Cell. 2011 Nov 11;147(4):728-41.
Mizushima N, Yoshimori T, Ohsumi Y.
The Role of Atg Proteins in Autophagosome Formation.
Annu Rev Cell Dev Biol. 2011 Nov 10;27:107-32.
Levine B, Mizushima N, Virgin H.W.
Autophagy in immunity and inflammation.
Nature 469: 323-335 (2011).
Mizushima N
Noboru Mizushima: All about autophagy. Interview by Caitlin Sedwick.
J Cell Biol. 2010 Sep 20;190(6):946-7.
Mizushima N, Levine B.
Autophagy in mammalian development and differentiation.
Nat Cell Biol. 2010 Sep;12(9):823-30.
Kuma A, Mizushima N.
Physiological role of autophagy as an intracellular recycling system: with an emphasis on nutrient metabolism.
Semin Cell Dev Biol. 2010 Sep;21(7):683-90. Epub 2010 Mar 17.
Mizushima, N.
Autophagy.
FEBS Lett. 2010 Apr 2;584(7):1279.
Mizushima, N., Yoshimori, T. and Levine, B.
Methods in mammalian autophagy research.
Cell 2010 Feb 5;140(3):313-26.
Mizushima, N.
The role of the Atg1/ULK1 complex in autophagy regulation.
Curr Opin Cell Biol. 2010 Apr;22(2):132-9. Epub 2010 Jan 6.
Stappenbeck TS, Rioux JD, Mizoguchi A, Saitoh T, Huett A, Darfeuille-Michaud A, Wileman T, Mizushima N, Carding S, Akira S, Parkes M, Xavier RJ.
Crohn disease: A current perspective on genetics, autophagy and immunity.
Autophagy. 2010 Feb 17;7(2).
Klionsky DJ, Codogno P, Cuervo AM, Deretic V, Elazar Z, Fueyo-Margareto J, Gewirtz DA, Kroemer G, Levine B, Mizushima N, Rubinsztein DC, Thumm M, Tooze SA.
A comprehensive glossary of autophagy-related molecules and processes.
Autophagy. 2010 May 6;6(4).
Mizushima, N.
Physiological functions of autophagy.
Curr Top Microbiol Immunol. 335: 71-84 (2009)
Ishihara, N. Mizushima, N.
A receptor for eating mitochondria.
Dev. Cell. 17: 1-2 (2009)
Nedjic, J., Aichinger, M., Mizushima, N., Klein, L.
Macroautophagy, endogenous MHC II loading and T cell selection: the benefits of breaking the rules.
Curr Opin Immunol. 21: 92-97 (2009)
Mizushima, N.
Methods for monitoring autophagy using GFP-LC3 transgenic mice.
Methods Enzymol. 2009;452:13-23.
Itakura, E., Mizushima, N.
Atg14 and UVRAG: Mutually exclusive subunits of mammalian Beclin 1-PI3K complexes.
Autophagy 5:534-536 (2009)
Hara, T., Mizushima, N.
Role of ULK-FIP200 complex in mammalian autophagy: FIP200, a counterpart of yeast Atg17?
Autophagy 5: 85-87 (2009)
Raben, N., Baum, R., Schreiner, C., Takikita, S., Mizushima, N., Ralston, E., Plotz, P.
When more is less: Excess and deficiency of autophagy coexist in skeletal muscle in Pompe disease.
Autophagy. 5: 111-113 (2009)
Tsukamoto, S., Kuma, A., Mizushima, N.
The role of autophagy during the oocyte-to-embryo transition.
Autophagy. 4: 1076-1078 (2008)
Mizushima, N., Kuma, A.
Autophagosomes in GFP-LC3 transgenic mice.
Methods Mol. Biol. 445: 119-124 (2008)
Mizushima, N., Levine, B., Cuervo, A.M., Klionsky, D.J.
Autophagy fights disease through cellular self-digestion
Nature 451:1069-1075 (2008)
Klionsky, D.J. et al.
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.
Autophagy 4:151-75 (2008)
Mizushima, N.
Autophagy: process and function
Genes Dev. 21: 2861-2873 (2007)
Mizushima, N., Yoshimori, T.
How to interpret LC3 immunoblotting
Autophagy 3:542-545 (2007)
Mizushima, N.
Collaboration of proteolytic systems.
Autophagy 3:179-180 (2007)
Mizushima, N., Klionsky, D.J.
Protein turnover via autophagy: Implications for metabolism.
Annu. Rev. Nutr. 27:19-40 (2007)
Mizushima, N., The pleiotropic role of autophagy: from protein metabolism to bactericide.
Cell Death Differ 12, 1535 1541 (2005).
Mizushima, N., Aβ generation in autophagic vacuoles.
J. Cell Biol. 171, 15-17 (2005).
Ohsumi, Y., Mizushima, N.
Two ubiquitin-like conjugation systems essential for autophagy.
Semin Cell Dev Biol. 15, 231-236 (2004).
Mizushima, N.
Methods for monitoring autophagy.
Int. J. Biochem. Cell Biol. 36, 2491-2502 (2004).
Mizushima, N., Yoshimori, T., Ohsumi, Y.
Role of the Apg12 conjugation system in mammalian autophagy.
Int. J. Biochem. Cell Biol. 35, 553-561 (2003).
Mizushima, N., Ohsumi, Y., Yoshimori, T.
Autohpagosome formation in mammalian cells.
Cell Struct. Funct. 27, 421-429 (2002).