ATCC Number: CRL-10741
Name: C3A; HepG2/C3A; derivative of Hep G2 (ATCC HB-8065)
Tissue: hepatoblastoma; liver; cancer
Species: human; male; Caucasian; 15 year old
Depositor: DEPOSITOR: Baylor College of Medicine - ASSIGNEE: Baylor
College of Medicine - INVENTOR: Kelly, JH
AnimalStrain:
Receptors: insulin; insulin like growth factor II (IGF II)
HeLaMarkers: no
VirusSuscept:
VirusResist:
Tumorigenic: no
Oncogene:
RevTranscript:
Karyotype:
Morphology: epithelial
Products: alpha-fetoprotein (alpha fetoprotein); albumin; alpha2
macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein) Growth: monolayer
Contaminants:
References: U.S. Pat. 5,290,684 (see ATCC HB-8065 for Hep G2
references) PassageSub:
Medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate 90%; fetal bovine serum, 10%. Newborn calf serum may be substituted for fetal serum Price Code: H
FreezeMedium: culture medium, 95%; DMSO, 5%
FluidRenewal: 2 times weekly
Subculturing: Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the flask to sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks
SplitRatio: A ratio of 1:4 to 1:6 is recommended
Comments: there is no evidence of a Hepatitis B virus genome in this cell line; C3A is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium; as the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion; gluconeogenesis activity is strongly oxgen dependent; the cells have nitrogen metabolizing activity comparable to perfused rat livers Note: This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims;