CYTOLOGIA Technical note

ON THE COVER

Vol. 73 No.3 September 2008

Technical note

     Metaphase chromosomes plate of male Hystrix brachyura Linnaeus, 1758 elucidate by G-banding technique.

     Malayan porcupine (H. brachyura) is a species of order Rodentia in family Hystricidae which have characteristic hard spines or quills. The morphology of Malayan porcupines are generally black, long spines or quills on lower back white with black band in middle, short spines on front parts of body mostly blackish, some with paler bases and tips. Spine along back of neck may from a crest. Tail has hollow, goblet-shaped quills that rattle when shaken. Malayan porcupines are found in south-east Asia including Thailand, Myanmar, Laos, Vietnam, Cambodia and Peninsular Malaysia. Also found in India, south China, Sumatra and Borneo. (Lekagul and McNeely 1988 Mammals of Thailand, 490: Francis 2008 A Field Guide to the Mammals of Thailand and South-East Asia, 378)
     This figure shown by Tanomtong et al in this issue (pp. 000–000) is the first chromosome report of H. brachyura. Diploid number are 2n66, the autosomes consist of 6 large submetacentric, 20 large acrocentric, 6 medium submetacentric, 18 medium acrocentric, 2 medium telocentric, 4 small submetacentric, 6 small acrocentric and 2 small telocentric chromosomes. In this investigation, the nucleolar organizer regions (NORs), which represents the chromosome marker, locates on the long arms of the pair submetacentric autosomes 9 and 13. The X chromosome was a large metacentric and the Y chromosome was the smallest telocentric chromosome. This result demonstrated that the chromosome band number of the H. brachyura from G-banding technique of metaphase chromosomes is 236 bands.

     (Alongkoad Tanomtong, Praween Supanuam, Wiwat Sangpakdee, Pronnarong Siripiyasing, Pawarisa Boonhan and Sarawut Kaewsri, Department of Biology, Facuty of Science, Khon Kaen University, Khon Kaen 40002, Thailand)

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ON THE COVER

Vol. 73 No.3 September 2008
Technical note

Metaphase chromosomes plate of male Hystrix brachyura Linnaeus, 1758 elucidate by G-banding technique. Malayan porcupine (H. brachyura) is a species of order Rodentia in family Hystricidae which have characteristic hard spines or quills. The morphology of Malayan porcupines are generally black, long spines or quills on lower back white with black band in middle, short spines on front parts of body mostly blackish, some with paler bases and tips. Spine along back of neck may from a crest. Tail has hollow, goblet-shaped quills that rattle when shaken. Malayan porcupines are found in south-east Asia including Thailand, Myanmar, Laos, Vietnam, Cambodia and Peninsular Malaysia. Also found in India, south China, Sumatra and Borneo. (Lekagul and McNeely 1988 Mammals of Thailand, 490: Francis 2008 A Field Guide to the Mammals of Thailand and South-East Asia, 378) This figure shown by Tanomtong et al in this issue (pp. 000–000) is the first chromosome report of H. brachyura. Diploid number are 2n66, the autosomes consist of 6 large submetacentric, 20 large acrocentric, 6 medium submetacentric, 18 medium acrocentric, 2 medium telocentric, 4 small submetacentric, 6 small acrocentric and 2 small telocentric chromosomes. In this investigation, the nucleolar organizer regions (NORs), which represents the chromosome marker, locates on the long arms of the pair submetacentric autosomes 9 and 13. The X chromosome was a large metacentric and the Y chromosome was the smallest telocentric chromosome. This result demonstrated that the chromosome band number of the H. brachyura from G-banding technique of metaphase chromosomes is 236 bands. (Alongkoad Tanomtong, Praween Supanuam, Wiwat Sangpakdee, Pronnarong Siripiyasing, Pawarisa Boonhan and Sarawut Kaewsri, Department of Biology, Facuty of Science, Khon Kaen University, Khon Kaen 40002, Thailand)
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