ON THE COVER

Vol. 73 No.1 Murch 2008

Technical note

     There shows metaphase chromosomes of 10 plant species. The magnification of each photo is equal and the bar represents 10μm.
     Ginkgo biloba(2n=24) 9751 Mbp/lC
     Cryptomeria japonica (2n = 22) 10824 Mpb/1C
     Rumex acetosa (male: 2n = 15) 1617 Mbp/1C
     Allium cepa (2n= 16) 16415 Mbp/1 C
     Vicia faba (2n = 12) 26852 Mbp/1C
     Hyacinthus orientalis (tetraploid: 2n = 32) 45668 Mbp/1C
     Punica granatum (2n = 16) 706 Mbp/1 C
     Limonium sinuatum (2n= 16) 3136 Mbp/1C
     Primula juriana hybrid (2n = 22) 490 Mbp/1C (Primula vulgaris)
     Plantago lanceolata (2n= 12) 490 Mbp/1C
     There is no C-value data in Primula juriana hybrid then shows the C-value of related species Primula vulgaris.
     C-value of each species is referring in Bennett M. D. and Leitch I. J., Plant DNA C-values Database, http ://data,kew.org/cvalues/database1.html.

     The root tips were treated with 0.50% colchicine before fixation. Each chromosome spread was made squashed after enzyme maceration with 0.5% pectolyase Y-23 and 2% cellulase Onozuka RS at 37°C for 20- 60mm. After preparation of chromosome spreads were air-dried and then were stained with 0.1μg/ml DAPTI in PBS, ph7.0. Fluorescence images were captured with chilled CCD camera and pseudo-color images were generated. Among plant species chromosome number, chromosome shapes and chromosome size are variable. The C-value is different in more than 100 times between Hyacinthus and Primula. The large difference in C-value is unique in plants; study of this phenomenon might bring important information for our understanding of chromosome evolution.

     (Fukashi Shibata¹ and Masahiro Hizume², ¹Division of Applied Bioscience, Graduate school of Agriculture, Hokkaido University, N9, W9, Kita-ku, Sapporo 060-8589, Japan,
²Faculty of Education, Ehime University Matsuyama 790-8577, Japan)

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ON THE COVER

Vol. 73 No.1 Murch 2008
Technical note

There shows metaphase chromosomes of 10 plant species. The magnification of each photo is equal and the bar represents 10μm. Ginkgo biloba(2n=24) 9751 Mbp/lC Cryptomeria japonica (2n = 22) 10824 Mpb/1C Rumex acetosa (male: 2n = 15) 1617 Mbp/1C Allium cepa (2n= 16) 16415 Mbp/1 C Vicia faba (2n = 12) 26852 Mbp/1C Hyacinthus orientalis (tetraploid: 2n = 32) 45668 Mbp/1C Punica granatum (2n = 16) 706 Mbp/1 C Limonium sinuatum (2n= 16) 3136 Mbp/1C Primula juriana hybrid (2n = 22) 490 Mbp/1C (Primula vulgaris) Plantago lanceolata (2n= 12) 490 Mbp/1C There is no C-value data in Primula juriana hybrid then shows the C-value of related species Primula vulgaris. C-value of each species is referring in Bennett M. D. and Leitch I. J., Plant DNA C-values Database, http ://data,kew.org/cvalues/database1.html. The root tips were treated with 0.50% colchicine before fixation. Each chromosome spread was made squashed after enzyme maceration with 0.5% pectolyase Y-23 and 2% cellulase Onozuka RS at 37°C for 20- 60mm. After preparation of chromosome spreads were air-dried and then were stained with 0.1μg/ml DAPTI in PBS, ph7.0. Fluorescence images were captured with chilled CCD camera and pseudo-color images were generated. Among plant species chromosome number, chromosome shapes and chromosome size are variable. The C-value is different in more than 100 times between Hyacinthus and Primula. The large difference in C-value is unique in plants; study of this phenomenon might bring important information for our understanding of chromosome evolution. (Fukashi Shibata¹ and Masahiro Hizume², ¹Division of Applied Bioscience, Graduate school of Agriculture, Hokkaido University, N9, W9, Kita-ku, Sapporo 060-8589, Japan, ²Faculty of Education, Ehime University Matsuyama 790-8577, Japan)
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