ON THE COVER

Vol. 72 No.1 March 2007

Technical note

A phase-contrast/immunofluorescence image of an isolated chloroplast division machinery including the PD, FtsZ and dynamin rings.

   Chloroplast division involves plastld-dividing(phase-contrast), dynamin (CmDum2/DRP5; orange) and FtsZ (CmFtsZ2; green)(PDF) rings. Isolated chloroplast divislon machineries (PDF machineries)from prmitive red alga Cyanidioschyzon merolae formed circular and supertwisted(or spiral) structure depending on the circumference or lengths. Supertwistedand spiral PDF machineries were longer than those of circular PDF-machineries.Thus, the supertwisted and circular PDF machineries were considered tobe derived from dividing chloroplasts at the early and late phases. respcctively.

    Because GTPase dynamin molecules are associated withthe outside of the PDF machinery and generate the motive force for contractionby the PD ring filament sliding in dividing chloroplast at the early phase,the isolatcd PDF machineries are supertwist structures. As the contractionof the PDF machineries progressed, the supertwisted (spiral) PDF machinerieschange into compact circles (cover image) and the dynamin molecules movefrom surface of the contracted PDF machinery to the insidc of the machinery.
When the dynamin molecules interact with the lipid membranes, they would act as a pinchase to pinch off the membranes at the bridge between the daughter chloroplasts.
[Yoshida, Y. et al. Isolated chloroplast divislon machinery can actively constrict after stretching. (2006) Science313, 1435-8.)]

     (Yamato Yoshida.^1,2 Haruko Kuroiwa,² Osami Misumi,1 Keiji Nishida,² Fumi Yasawa,² Takayuki Fujiwara,³ Hideaki Nanamiya^,2 Fujio Kawamura,^2,3 Tsuneyoshi Kuroiwa², ¹ Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo 277-8562, Japan, 2 Research Information Center for Extremophilc, 3 Laboratory of Cell Biology, Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Toshima, Tokyo 171-8501, Japan)

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ON THE COVER

Vol. 72 No.1 March 2007
Technical note

A phase-contrast/immunofluorescence image of an isolated chloroplast division machinery including the PD, FtsZ and dynamin rings. Chloroplast division involves plastld-dividing(phase-contrast), dynamin (CmDum2/DRP5; orange) and FtsZ (CmFtsZ2; green)(PDF) rings. Isolated chloroplast divislon machineries (PDF machineries)from prmitive red alga Cyanidioschyzon merolae formed circular and supertwisted(or spiral) structure depending on the circumference or lengths. Supertwistedand spiral PDF machineries were longer than those of circular PDF-machineries.Thus, the supertwisted and circular PDF machineries were considered tobe derived from dividing chloroplasts at the early and late phases. respcctively. Because GTPase dynamin molecules are associated withthe outside of the PDF machinery and generate the motive force for contractionby the PD ring filament sliding in dividing chloroplast at the early phase,the isolatcd PDF machineries are supertwist structures. As the contractionof the PDF machineries progressed, the supertwisted (spiral) PDF machinerieschange into compact circles (cover image) and the dynamin molecules movefrom surface of the contracted PDF machinery to the insidc of the machinery. When the dynamin molecules interact with the lipid membranes, they would act as a pinchase to pinch off the membranes at the bridge between the daughter chloroplasts. [Yoshida, Y. et al. Isolated chloroplast divislon machinery can actively constrict after stretching. (2006) Science313, 1435-8.)] (Yamato Yoshida.^1,2 Haruko Kuroiwa,² Osami Misumi,1 Keiji Nishida,² Fumi Yasawa,² Takayuki Fujiwara,³ Hideaki Nanamiya^,2 Fujio Kawamura,^2,3 Tsuneyoshi Kuroiwa², ¹ Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo 277-8562, Japan, 2 Research Information Center for Extremophilc, 3 Laboratory of Cell Biology, Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Toshima, Tokyo 171-8501, Japan)
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