ON THE COVER

Vol. 69 No.2 June 2004

Technical note

     In the final step of' mitochondrial division, outer and inner division apparatuses are loosely associated in a red alga Cyanidioschyzon merolae.

     Mitochondrial matrix (red), FtsZ ring (green), and Dynamin ring (blue) were respectively immunolabeled with the antisera against EF-Tu(mt), CmFtsZl or CmDnml,and visualized with secondary antibodies conjugated with Alexa555, Alexa488 or lexa350. Images were observed under a fluorescence microscopy and digitally captured and reproduced through a cooled 3CCD camera system. The phase contrast image was faintly overlaid on the merged image. Bar: 1µm
     While mitochondrial FtsZ is thought to maintain the placement of mitochondrial division site at inside of the inner membrane, dynamin is recruited for the final severance of mitochondria to the mitochondrion dividing ring (MD ring) at the outside of the constricted outer membrane. The fact that the localizations of the FtsZ (green) and the dynamin (blue) do not tightly associated with each other suggest that the MD ring but not FtsZ mediates the placement of the final severance diven by dynamin (see Nishida K., Misumi O., Yagisawa F., Kuroiwa H., Nagata T. and Kuroiwa T. 2004: J Histochem. Cytochem., 52(7): 843-849).

     (Keiji Nishida, Department of Life Science, Rikkyo Collage Univcrsity. Nishiikebukuro, Toshima-ku, Tokyo, 171 - 8501, Japan)

Back      Archive list        English Top Japanese Top

ON THE COVER

Vol. 69 No.2 June 2004
Technical note

In the final step of' mitochondrial division, outer and inner division apparatuses are loosely associated in a red alga Cyanidioschyzon merolae. Mitochondrial matrix (red), FtsZ ring (green), and Dynamin ring (blue) were respectively immunolabeled with the antisera against EF-Tu(mt), CmFtsZl or CmDnml,and visualized with secondary antibodies conjugated with Alexa555, Alexa488 or lexa350. Images were observed under a fluorescence microscopy and digitally captured and reproduced through a cooled 3CCD camera system. The phase contrast image was faintly overlaid on the merged image. Bar: 1µm While mitochondrial FtsZ is thought to maintain the placement of mitochondrial division site at inside of the inner membrane, dynamin is recruited for the final severance of mitochondria to the mitochondrion dividing ring (MD ring) at the outside of the constricted outer membrane. The fact that the localizations of the FtsZ (green) and the dynamin (blue) do not tightly associated with each other suggest that the MD ring but not FtsZ mediates the placement of the final severance diven by dynamin (see Nishida K., Misumi O., Yagisawa F., Kuroiwa H., Nagata T. and Kuroiwa T. 2004: J Histochem. Cytochem., 52(7): 843-849). (Keiji Nishida, Department of Life Science, Rikkyo Collage Univcrsity. Nishiikebukuro, Toshima-ku, Tokyo, 171 - 8501, Japan)
Back Archive list English Top Japanese Top