From Bench to Bedside, and Beyond
Index
From Bench to Bedside, and Beyond
Classical view of lipids
New view of lipids
Eicosanoids
Roles of eicosanoids in human
How asprin works ?
Inappropriate usage of aspirin causes severe gastric ulcer
Why are steroids more potent anti-inflammatory drug than aspirin?
What are leukotrienes?
Inflammatory response and chemotaxis
Leukotriene B4 is the most potent chemoattractant
Structure of human leukotrien B4 receptor
Boyden chamber assay
Rat ischemic acute renal faulure (iARF) model
Serum Cr and BUN in rat iARF
Myeloperoxidase (MPO) activity in kidney
BLT antagonist inhibits neutrophil infiltration
BLT antagonist improves renal functions
Discovery of the second LTB4 receptor
Leukotriene receptors
Among many phospholipase A2 (PLA2), why is cPLA2 important?
To see the biological roles of cPLA2 in vivo, we made cPLA2 gene-knockout mice.
Asthmatic symptoms are markedly reduced in cPLA2 knockout mice
ARDS (Adult respiratory distress syndrome)
Chest X-ray of ARDS patient
Change in Lung Elastance of cPLA2 Deficient Mice after LPS/Zymosan-Administration
Lung Tissues after Sepsis-ARDS
cPLA2 is induced in intestinal polyps of Apc KO mice, a model for colon cancer
Colon polyp is much smaller in cPLA2 knockout mice
Much milder symptoms in collagen-induced arthritis
cPLA2-deficient mice showed a marked decrease in allergic responses, etc.
Unexpected observations (1)
Unexpected observations (2)
Lipid mediators as a major target for the next decade research
Two supervisors of my research
Collaborators
University of Tokyo
Famous novelist and MD, Ogai Mori